Surface-enhanced Raman scattering (SERS) is widely employed for amino acids quantitative analysis due to its high-sensitivity molecular feature extraction and multiplex detection advantage. Stable and highly sensitive SERS substrates are crucial for achieving precise detection. Gold core silver shell nanoparticles (Au@Ag NPs) combine the chemical stability of gold nanoparticles with the high SERS enhancement effect of silver nanoparticles. They enable the screening and detection of seven amino acids, including phenylalanine and alanine. Except for isoleucine and glycine, which exhibited relatively lower sensitivity, the detection limits for the remaining amino acids reached 1×10^-4$ \mathrm{m}\mathrm{o}\mathrm{l}/\mathrm{L} $. A linear correlation existed between concentration and SERS signal intensity, with correlation coefficients exceeding 0.94. This study provides a reference method for simultaneous quantitative detection of multiple amino acids on a single substrate.