A novel inclusion compound ［-OOCCH2N+(CH3)2］2(CH2)3·4(NH2)2CS has been prepared with thiourea and double betaine, and the crystal structure was characterized by single crystal X-ray diffraction. Crystal structural analysis shows that the complex is triclinic, with space group P1, a=0.884 5(2) nm, b=0.936 7(2) nm, c=0.946 4(3) nm, α=91.591(2)°, β=91.591(2)°, γ=91.591(2)°, Z=1, R1=0.039 9, wR2=0.100 6(I>2σ(I)). In the crystal structure of the title compound, thiourea molecules are connected side by side through N-H…S hydrogen bonds to form a tetramer. The hydrogen bond layer is formed by carboxyl groups of the guest molecules and the thiourea tetramer through N-H…O hydrogen bonds. And the guest dications are sandwiched between adjacent hydrogen bonding layers to form a sandwich crystal structure.