In this paper, the results showed that single carotenoid and their combinations significantly reduced LDH activity and MDA content (P<0.05), and had protective effects on UVA-induced oxidative damage to HSF cells. At the same concentration, astaxanthin had better LDH reduction activity than β-carotene, and had better protective effect on oxidative damage of HSF cells. This study provides a theoretical basis for the development of cosmetics and health care products with the protective effect on UVA radiation oxidative damage with carotenoids.