We present a novel spectrum-resolved multifocal multiphoton microscopy (SR-MMM) that is capable of performing fast 2-dimensional (2D) spectral measurements of fluorescent samples with optical sectioning. Fluorescence emission from the excited lines on the sample is spectrally dispersed with a prism,and a CCD array is used to acquire the spectrally resolved image. Spectrally resolved images of 512 pixel×512 pixel can be obtained by acquiring only 128 raw images when a 4×4 excitation foci array is used. Multi-color fluoresent beads,sample slide of a stained lily and pollen particles are used for spectrally resolved imaging experiment. The spectral measurement range is from 450 nm to 700 nm,and the spectral resolution is less than 3 nm.