Acta Laser Biology Sinica, Volume. 31, Issue 2, 188(2022)

Extraction Method and Quality Identification of Genomic DNA from Human Tissue Samples Soaked in Formaldehyde Solution

YANG Boyu1, CAI Yi1, SUN Luning1, LUAN Zedong2, PANG Wenyan2, WU Xiushan1, FAN Xiongwei1, and JIANG Zhigang1、*
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  • 1[in Chinese]
  • 2[in Chinese]
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    Human tissue samples soaked in formaldehyde solution are rare resources in many cases, and the extraction of their DNA is a challenging issue. In this paper, genomic DNA was extracted from four formalin soaked human embryo skin and muscle samples by using an improved method for extracting genomic DNA from samples soaked in formaldehyde solution and a generic genomic DNA extraction kit. The purity and concentration of extracted DNA were determined by UV spectrophotometry, and the DNA solution was subjected to agarose gel electrophoresis and imaging. The results showed that the concentration and purity of genomic DNA obtained by the improved extraction method were better than those obtained by the general method. PCR amplification was carried out with the extracted genomic DNA template. Electrophoresis results showed that the improved extraction method had better PCR amplification effect. Our study provides methodological guidance and a reference for the direction of improvement for the extraction of genomic DNA from precious samples soaked in formaldehyde solution.

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    YANG Boyu, CAI Yi, SUN Luning, LUAN Zedong, PANG Wenyan, WU Xiushan, FAN Xiongwei, JIANG Zhigang. Extraction Method and Quality Identification of Genomic DNA from Human Tissue Samples Soaked in Formaldehyde Solution[J]. Acta Laser Biology Sinica, 2022, 31(2): 188

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    Paper Information

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    Received: Feb. 7, 2022

    Accepted: --

    Published Online: Jul. 25, 2022

    The Author Email: JIANG Zhigang (270212729@qq.com)

    DOI:10.3969/j.issn.1007-7146.2022.02.013

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