The composite film of reduced graphene oxide/TiO2 nanowires(rGO/TiO2 NWs) was prepared by two-step hydrothermal method united vacuum filtration using graphene and self-prepared TiO2 powder as raw materials. The sample was characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffractometer (XRD) and Raman spectroscopy. The results show that reduced graphene oxide and TiO2 NWs are successfully compounded, and the dispersion of TiO2 NWs are good. The adsorption experiments of Cu2+ show that the proportion of TiO2 NWs in the composite, pH values are the important factors affecting the Cu2+ adsorption, the adsorption of Cu2+on the composite should be studied near neutral environment with pH=6.0. While containing 50% TiO2 NWs, the adsorption capacity of the composite film for Cu2+ is 4 times of rGO film. The composite film has good adsorption stability, and the adsorption rate still retains 91% of the original adsorption after 5 times of reuse.