Herein, we proposed a dynamic target scattering imaging method. First, we measured the optical transmission matrix by using a four-step phase-shifting interferometry method. Then, we performed numerical simulations of tracking and reconstruction for different dynamic targets through the scattering medium based on phase conjugation, Tikhnov regularization, and total variation minimization algorithms. The feasibility of this method was experimentally verified, and the properties of the three algorithms were compared. The results show that the antinoise ability of the total variation minimization algorithm is the best. The proposed method provides a new idea for dynamic target imaging through scattering media in the biomedical field.