To find out the role of laser parameters on in-vitro tissue bonding, experiments are designed to study the effect of laser parameters, such as power and scanning mode, on the appearance and tensile strength of in-vitro skin tissue incision, and then the process parameters are optimized. Results show that the appearance and tensile strength of tissue incision is better and greater when we use low laser power with long welding time, and also the irreversible thermal damage decreases. Using interval laser scanning increases the bioactivity closed to the incision and decreases the thermal damage. Experiments are performed to verify the reliability and stability of the optimized parameters, and the tensile strength of incision is tested. Results show that the bonding along the depth of tissue can be realized and no carbide or burning occurs. Compared with that of the continuous laser welding process, the welding time is decreased by 30%-40%, and the tensile strength of incision is 0.38 MPa, which can meet the requirement.