Olfactory related proteins of insects play an important role in their behaviors and physiological activities such as foraging, mating, defense, spawning, information exchange and habitat selection. Fluorescence competitive binding assay is a typical method for detecting the binding properties of insect OBPs and CSPs with environmental odor molecules. In this paper, some researches and applications on fluorescence competitive binding assay used for insects olfactory proteins (OBPs and CSPs) are reviewed. It provides theoretical reference for further exploration on insects olfactory recognition mechanism and formulation strategies on pests control and economic insects protection.