Thermoelectric materials can realize the direct conversion between heat energy and electric energy. BaBi2Se4 becomes a potential thermoelectric material because of its low thermal conductivity and good electrical conductivity. In this paper, BaBi2Se4 was selected as a research object, and the phase composition, thermal and electronic properties were investigated. The results show that all the samples exhibit N-type semiconductor conductivity characteristics. Te-alloying can inhibit Bi2Se3 impurities in the samples and decrease the carrier concentration, thus optimizing the electronic transport performance. Also, the lattice thermal conductivity is low due to the complex crystal structure. These results indicate that BaBi2Se4 is a potential thermoelectric material.