Spectroscopy and Spectral Analysis, Volume. 40, Issue 11, 3477(2020)

Detection of Protease Deterioration Factor in Tomato by Fluorescence Sensor Array

Meng-yao LI1...1,*, Shu-ya WANG1,1, Yun-feng XIE1,1, Yun-guo LIU1,1, and Chen ZHAI11 |Show fewer author(s)
Author Affiliations
  • 1[in Chinese]
  • 11. Nutrition & Health Research Institute, COFCO Corporation, Beijing Key Laboratory of Nutrition & Health and Food Safety, Beijing 102209, China
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    Figures & Tables(4)
    (a) Principle of fluorescence immunoassay; (b) Synthesis of probe and its reaction with carboxylesterase; (c) Schematic of simultaneous detection of multiple enzymes
    (a) Fluorescence spectra and linear fitting curve of pectinases at different concentrations; (b) Fluorescence spectra of probe (10 μmol·L-1) reacting with carboxylesterase at different concentrations
    Fluorescence responses of immune-fluorescent probes with other interferences
    • Table 1. Addition and recycling experiment of sample (n=5)

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      Table 1. Addition and recycling experiment of sample (n=5)

      酶种类加标量/
      (U·mL-1)
      检测平均值/
      (U·mL-1)
      标准
      偏差
      回收率
      /%
      变异系
      数/%
      PE0.1
      100.0
      300.0
      0.09
      92.1
      277.6
      0.006
      10.4
      23.1
      90.0
      92.1
      92.5
      6.7
      11.3
      8.3
      CAT0.2
      400.0
      600.0
      0.18
      376.0
      568.0
      0.008
      22.9
      45.7
      90.0
      94.0
      94.7
      4.4
      6.1
      8.1
      SOD1.0
      500.0
      600.0
      0.95
      483.0
      572.0
      0.1
      41.5
      47.9
      95.0
      96.6
      95.3
      10.5
      8.6
      8.4
      PPO20.0
      30.0
      50.0
      20.2
      30.6
      49.8
      2.7
      3.1
      3.9
      101.1
      102.3
      99.7
      13.4
      10.1
      7.8
      CXE0.15
      0.2
      0.3
      0.14
      0.18
      0.28
      0.012
      0.017
      0.023
      93.3
      98.0
      93.0
      8.6
      9.1
      8.0
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    Meng-yao LI, Shu-ya WANG, Yun-feng XIE, Yun-guo LIU, Chen ZHAI. Detection of Protease Deterioration Factor in Tomato by Fluorescence Sensor Array[J]. Spectroscopy and Spectral Analysis, 2020, 40(11): 3477

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    Paper Information

    Category: Research Articles

    Received: Oct. 29, 2019

    Accepted: --

    Published Online: Jun. 18, 2021

    The Author Email: LI Meng-yao (Mengyaolik@163.com)

    DOI:10.3964/j.issn.1000-0593(2020)11-3477-06

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