Laser & Optoelectronics Progress, Volume. 56, Issue 6, 062601(2019)

Protein Detection Chip Based on Quantum Dot Fluorescence Quenching Method

Man Peng1,3, Zhongchen Bai2, Ying Zhang2, Xianji Li2, and Zhengping Zhang2、*
Author Affiliations
  • 1 College of Big Data and Information Engineering, Guizhou University, Guiyang, Guizhou 550025, China
  • 2 Key Laboratory for Photoelectronics and Application Technology, Guizhou University, Guiyang, Guizhou 550025, China
  • 3 School of Mechanical & Electrical Engineering, Guizhou Normal University, Guiyang, Guizhou 550025, China;
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    Figures & Tables(8)
    Principle of protein-chip detection. (a) Chip structure; (b) detection principle
    Experimental setup. (a) Block diagram; (b) prototype photo
    AFM of glass substrate. (a) Unmodified; (b) modified
    Infrared Fourier transform spectrum of glass substrate modified by APTES
    Nanometer quantum dot biochip array. (a) Surface, the inset is an array unit photo; (b) fluorescence, the inset is a fluorescent image
    Nanometer quantum dot biochip array unit. (a)-(c) Photos; (d)-(f) fluorescent images after binding antibodies; (g)-(i) fluorescent images after binding antigens on antibody substrate
    Relationship between NGF concentration and fluorescence peak intensity. (a) 0-100 ng·mL-1; (b) partial enlargement
    • Table 1. Fluorescence quenchingdegrees under different antigen concentrations

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      Table 1. Fluorescence quenchingdegrees under different antigen concentrations

      No.12345678910111213
      Concentration ofAg-V /(ng·mL-1)0.10.20.51.02.55.07.510.012.517.520.050.0100.0
      FQD /arb. units6.52.6517.773.8545.68126.68143.76158.16205.68273.72344.28447.84454.72
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    Man Peng, Zhongchen Bai, Ying Zhang, Xianji Li, Zhengping Zhang. Protein Detection Chip Based on Quantum Dot Fluorescence Quenching Method[J]. Laser & Optoelectronics Progress, 2019, 56(6): 062601

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    Paper Information

    Category: Physical Optics

    Received: Aug. 15, 2018

    Accepted: Sep. 30, 2018

    Published Online: Jul. 30, 2019

    The Author Email: Zhang Zhengping (pengman1001@163.com)

    DOI:10.3788/LOP56.062601

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