Improving signal-to-background ratio by orders of magnitude in high-speed volumetric imaging <i>in vivo</i> by robust Fourier light field microscopy

Jiazhen Zhai; Ruheng Shi; Lingjie Kong

doi:10.1364/PRJ.451895

Photonics Research, Volume. 10, Issue 5, 1255(2022)

Improving signal-to-background ratio by orders of magnitude in high-speed volumetric imaging in vivo by robust Fourier light field microscopy

Jiazhen Zhai^1、†, Ruheng Shi^1、†, and Lingjie Kong^1,2、*

Author Affiliations

¹State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China

²IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing 100084, China

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Supplementary Materials

Visualization 1 | Neuron activity in brains of larval zebrafish in vivo. (a). FLFM reconstructed images, at z = -30, 0, 30 µm from left to right columns, respectively. (b). RFLFM reconstructed images, at z = -30, 0, 30 µm from left to right columns, respectively. The Sc

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Visualization 2 | Heart-beating imaging of larval zebrafish (RFLFM mode) in vivo. Scale bar: 100 µm. The video is played with original speed.

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Visualization 3 | Vascular dilations in mouse brains in vivo. (a). FLFM reconstructed images, we choose ROIs of 670×460 µm2 at z=0, 22.5, 36 µm from left to right columns, respectively. (b). RFLFM reconstructed images, the same ROIs as Fig. S10a, respectively. The Sca

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Visualization 4 | Neuronal network activity in mouse brains in vivo. (a). RFLFM reconstructed images. (b). FLFM reconstructed images. The Scale bar: 200 µm. The MIP images are displayed in gray as a constant and the videos are displayed in color. The video is played a

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Jiazhen Zhai, Ruheng Shi, Lingjie Kong. Improving signal-to-background ratio by orders of magnitude in high-speed volumetric imaging in vivo by robust Fourier light field microscopy[J]. Photonics Research, 2022, 10(5): 1255

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Paper Information

Category: Imaging Systems, Microscopy, and Displays

Received: Dec. 21, 2021

Accepted: Mar. 16, 2022

Published Online: Apr. 20, 2022

The Author Email: Lingjie Kong (konglj@tsinghua.edu.cn)

DOI:10.1364/PRJ.451895

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology