Laser & Optoelectronics Progress, Volume. 60, Issue 22, 2210005(2023)

Multi-Module Combination Method for Denoising Biological Microscopy Images

Weihua Le, Dingrong Yi*, Bocong Zhou, and Caihong Huang
Author Affiliations
  • College of Mechanical Engineering and Automation, Huaqiao University, Xiamen 361021, Fujian , China
  • show less
    Figures & Tables(12)
    Overall structure of multi-module combined denoising network model
    Structure design of channel association module, adopting different structures in the first and last layers, but the same structure in the middle layers
    Structural design of the multi-scale denoising module, including the information extraction layer of different scale convolution kernels, the middle U-Net layer and the upsampling layer of different magnifications
    Structure of fusion compression module
    Examples of scanned areas of animal and plant samples and their corresponding Ground Truth images. (a) Slice of Cyclops; (b) hair follicle slice; (c) lichen slice; (d) stem longitudinal cut of terminal apex
    Changes of some parameters during network model training and recovery performance at different stages of training. (a) Change trend of loss value of network model in the training set and accuracy in the verification set; (b) recovery effect of a randomly selected image at different stages of network model training
    Visual effect of cross-section image data of bluegrass leaves processed by different denoising methods. (a) Noise image; (b) repair result of Gaussian filter; (c) repair result of BM3D; (d) repair result of DnCNN; (e) repair result of FFDNet; (f) repair result of CBDNet; (g) repair result of DNMMC
    Visual effect of image data of fish gill cross-section samples processed by different denoising methods. (a) Noise image; (b) repair result of Gaussian filter; (c) repair result of BM3D; (d) repair result of DnCNN; (e) repair result of FFDNet; (f) repair result of CBDNet; (g) repair result of DNMMC
    Visual effect of image data of spore cup samples processed by different denoising methods. (a) Noise image; (b) repair result of Gaussian filter; (c) repair result of BM3D; (d) repair result of DnCNN; (e) repair result of FFDNet; (f) repair result of CBDNet; (g) repair result of DNMMC
    • Table 1. PSNR average results of different denoising algorithms for 10 images in the real cell noise image benchmark set

      View table

      Table 1. PSNR average results of different denoising algorithms for 10 images in the real cell noise image benchmark set

      Image No.Gaussian filterBM3DDnCNNFFDNetCBDNetOurs
      124.165125.822427.987227.610727.252430.2281
      227.842129.203529.276129.436329.756331.5100
      321.866125.097827.888328.424727.145230.8424
      421.386026.232828.091728.782828.488531.2100
      521.443824.985027.352527.030926.749029.4740
      622.167526.477028.190129.043328.574831.3430
      725.048328.156829.313629.824630.417632.1973
      821.454124.022126.836026.197525.407329.3209
      924.455828.352929.033129.964130.155131.9401
      1024.679428.383929.212430.084430.292032.2160
    • Table 2. SSIM average results of different denoising algorithms for 10 images in the real cell noise image benchmark set

      View table

      Table 2. SSIM average results of different denoising algorithms for 10 images in the real cell noise image benchmark set

      Image No.Gaussian filterBM3DDnCNNFFDNetCBDNetOurs
      10.56790.61930.75060.69150.69430.8271
      20.63920.68200.68810.68600.71350.7756
      30.64110.76400.76140.85480.84100.9094
      40.70800.84340.81120.89260.88990.9290
      50.67720.79840.85350.85880.85480.9156
      60.71880.83510.76830.87570.87690.9073
      70.70500.78620.75640.82070.86060.8828
      80.57240.67290.81700.77320.74430.8853
      90.70750.80990.79310.84260.85770.8880
      100.71550.80980.74520.83700.85600.8824
    • Table 3. Average running time of 10 randomly selected images processing by different denoising methods

      View table

      Table 3. Average running time of 10 randomly selected images processing by different denoising methods

      RepeatGaussian filterBM3DDnCNNFFDNetCBDNetOurs
      145.27963.344113.42261.000243.3593.226
      245.92463.281115.06361.953246.2973.240
      345.92263.969112.01658.500235.7973.222
      445.43363.531113.42263.219237.9533.236
      545.72263.484112.43863.313242.6723.192
    Tools

    Get Citation

    Copy Citation Text

    Weihua Le, Dingrong Yi, Bocong Zhou, Caihong Huang. Multi-Module Combination Method for Denoising Biological Microscopy Images[J]. Laser & Optoelectronics Progress, 2023, 60(22): 2210005

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category: Image Processing

    Received: Apr. 10, 2023

    Accepted: Jun. 15, 2023

    Published Online: Nov. 6, 2023

    The Author Email: Yi Dingrong (yidr@hqu.edu.cn)

    DOI:10.3788/LOP231054

    Topics