Photonics Research, Volume. 6, Issue 9, 918(2018)
Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films
Fig. 1. Schematic diagram to control the refractive index of DNA thin solid film by denaturation. Denaturation is activated by adding NaOH in DNA aqueous solution precursors, which is irreversibly immobilized in thin solid film to change the refractive index (ds, double stranded; ss, single stranded).
Fig. 2. DNA thin solid film fabrication process. (a) DNA aqueous solution and DNA-NaOH aqueous solution where the denaturation is reversible. (b)
Fig. 3. (a) UV/visible spectra of DNA aqueous solution with various NaOH concentrations in the precursor solutions. Here we used 0.15 wt. % DNA. (b) Hyperchromicity near
Fig. 4. (a) FTIR absorption spectra of solid freestanding DNA films made from DNA aqueous solutions with various NaOH concentrations. (b) Spectral shift of the cytosine vibration peak as a function of NaOH concentration in the DNA precursor solutions.
Fig. 5. (a) The refractive indices of DNA thin solid film in the spectral range from 380 to 900 nm for various NaOH concentrations in precursor solutions. (b) The refractive indices of DNA thin solid film as a function of NaOH concentration in the precursor solutions.
Fig. 6. Thermally induced changes in the refractive index and the film thickness of DNA thin solid film with various NaOH concentrations in precursor solutions. (a) Refractive index at
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Hayoung Jeong, Paulson Bjorn, Seongjin Hong, Seunguk Cheon, Kyunghwan Oh. Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films[J]. Photonics Research, 2018, 6(9): 918
Category: Medical Optics and Biotechnology
Received: May. 14, 2018
Accepted: Jul. 26, 2018
Published Online: Sep. 5, 2018
The Author Email: Kyunghwan Oh (koh@yonsei.ac.kr)