Photonics Research, Volume. 6, Issue 9, 918(2018)

Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films

Hayoung Jeong, Paulson Bjorn, Seongjin Hong, Seunguk Cheon, and Kyunghwan Oh*
Author Affiliations
  • Photonic Device Physics Laboratory, Institute of Physics and Applied Physics, Yonsei University, 50 Yonsei-ro Seodaemun-gu, Seoul 120-749, South Korea
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    Figures & Tables(8)
    Schematic diagram to control the refractive index of DNA thin solid film by denaturation. Denaturation is activated by adding NaOH in DNA aqueous solution precursors, which is irreversibly immobilized in thin solid film to change the refractive index (ds, double stranded; ss, single stranded).
    DNA thin solid film fabrication process. (a) DNA aqueous solution and DNA-NaOH aqueous solution where the denaturation is reversible. (b) O2 plasma treatment on Si/SiO2 substrate to make hydrophilic surfaces. (c) Dispensing aqueous solution precursors on the substrate. (d) Spinning and solidification by water evaporation. (e) Single-stranded DNAs are maintained in the thin solid film to achieve the irreversible denaturation.
    (a) UV/visible spectra of DNA aqueous solution with various NaOH concentrations in the precursor solutions. Here we used 0.15 wt. % DNA. (b) Hyperchromicity near λ=260 nm was clearly observed by increasing the NaOH concentration.
    (a) FTIR absorption spectra of solid freestanding DNA films made from DNA aqueous solutions with various NaOH concentrations. (b) Spectral shift of the cytosine vibration peak as a function of NaOH concentration in the DNA precursor solutions.
    (a) The refractive indices of DNA thin solid film in the spectral range from 380 to 900 nm for various NaOH concentrations in precursor solutions. (b) The refractive indices of DNA thin solid film as a function of NaOH concentration in the precursor solutions.
    Thermally induced changes in the refractive index and the film thickness of DNA thin solid film with various NaOH concentrations in precursor solutions. (a) Refractive index at λ=633 nm as a function of temperature in the first temperature cycle; (b) refractive index at λ=633 nm as a function of temperature in the second cycle. (c) Film thickness as a function of temperature in the first cycle; (d) film thickness as a function of temperature in the second cycle.
    • Table 1. Average Thickness of DNA Thin Solid Films Made from Precursor Solutions with Various NaOH Concentrations

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      Table 1. Average Thickness of DNA Thin Solid Films Made from Precursor Solutions with Various NaOH Concentrations

      NaOH in Precursor Solution (mM)Thin Film Thickness (nm)
      043.8±0.6
      2.538.6±2.3
      5.042.8±3.4
      7.539.6±1.6
    • Table 2. Thermo-Optic Coefficient of DNA Thin Solid Film at a Wavelength of 633  nm for Various NaOH Concentrations in the Precursor Solutions

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      Table 2. Thermo-Optic Coefficient of DNA Thin Solid Film at a Wavelength of 633  nm for Various NaOH Concentrations in the Precursor Solutions

      NaOH Concentration in Precursor Solution (mM)dn/dT (1st Cycle, 104  °C1)dn/dT (2nd Cycle, 104  °C1)
      0−4.06−3.86
      2.5−4.66−4.37
      5.0−5.06−4.76
      7.5−5.51−5.24
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    Hayoung Jeong, Paulson Bjorn, Seongjin Hong, Seunguk Cheon, Kyunghwan Oh. Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films[J]. Photonics Research, 2018, 6(9): 918

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    Paper Information

    Category: Medical Optics and Biotechnology

    Received: May. 14, 2018

    Accepted: Jul. 26, 2018

    Published Online: Sep. 5, 2018

    The Author Email: Kyunghwan Oh (koh@yonsei.ac.kr)

    DOI:10.1364/PRJ.6.000918

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