Laser & Optoelectronics Progress, Volume. 58, Issue 18, 1811023(2021)

Polarization Microscopy Imaging of Biological Cells Based on Mueller Matrix

Ruijuan Gao, Chunhua Wang*, Jinxing Ning, Keshuai Wu, and Xiang Gu
Author Affiliations
  • Key Laboratory of Specialty Fiber Optics and Optical Access Networks, Shanghai University, Shanghai 200444, China
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    Figures & Tables(10)
    Principle of the Mueller matrix polarization microscopy imaging system
    Four uncorrelated PSG polarization states
    Measured and theoretical values of the Mueller matrix
    Normalized Mueller matrix imaging results of the inner epidermal tissue of onion. (a) Mass fraction is 0%; (b) mass fraction is 2.5%; (c) mass fraction is 5%; (d) mass fraction is 10%
    Mueller matrix elements of onion inner epidermal tissue under different mass fractions of glucose solutions. (a) m22; (b) m23
    Mueller matrix polarization decomposition parameters of onion inner epidermal tissue in glucose solutions with different mass fractions. (a) Intensity; (b) depolarization; (c) circular retardance; (d) diattenuation
    Imaging results of human laryngeal carcinoma cells at different experimental time. (a) Intensity imaging; (b) Mueller matrice
    Mueller matrix polarization decomposition imaging of human laryngeal carcinoma cells at different experimental time. (a) Depolarization; (b) circular retardance; (c) diattenuation
    • Table 1. Collimation direction of the optical device unit: (°)

      View table

      Table 1. Collimation direction of the optical device unit: (°)

      Polarization statePSGPSA
      P1HWP1QWP1QWP2P2
      SH00000
      SL00-45045
      SR00454545
      SP022.5459045
    • Table 2. Calculation formulae of the Mueller matrix elements

      View table

      Table 2. Calculation formulae of the Mueller matrix elements

      ElementFormula
      m11ILL+ILP+IRL+IRP
      m12-ILL-ILP-IRL-IRP+2IHL+2IHP
      m13-ILL-ILP-IRL-IRP+2IPL+2IPP
      m14-ILL-ILP+IRL+IRP
      m21-ILL-ILP-IRL-IRP+2ILH+2IRH
      m22ILL+ILR+IRL+IRP-2ILH-2IRH-2IHL-2IHP+4IHH
      m23ILL+ILP+IRL+IRP-2ILH-2IRH-2IPL-2IPP-4IPH
      m24ILL+ILP-IRL-IRP-2ILH+2IRH
      m31-ILL-ILP-IRL-IRP+2ILR+2IRR
      m32ILL+ILP+IRL+IRP-2ILR-2IRR-2IHL-2IHP+4IHR
      m33ILL+ILP+IRL-IRP-2ILR-2IRR-2IPL-2IPP+4IPR
      m34ILL+ILP-IRL-IRP-2ILR+2IRR
      m41ILL-ILP+IRL-IRP
      m42-ILL+ILP-IRL+IRP+2IHL-2IHP
      m43-ILL+ILP-IRL+IRP+2IPL-2IPP
      m44-ILL+ILP+IRL-IRP
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    Ruijuan Gao, Chunhua Wang, Jinxing Ning, Keshuai Wu, Xiang Gu. Polarization Microscopy Imaging of Biological Cells Based on Mueller Matrix[J]. Laser & Optoelectronics Progress, 2021, 58(18): 1811023

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    Paper Information

    Category: Imaging Systems

    Received: Apr. 20, 2021

    Accepted: Jun. 30, 2021

    Published Online: Aug. 28, 2021

    The Author Email: Wang Chunhua (lizawch@staff.shu.edu.cn)

    DOI:10.3788/LOP202158.1811023

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