Spectroscopy and Spectral Analysis, Volume. 42, Issue 3, 821(2022)
Spectroscopic Study on the Mechanism of Photoduction of Cytochrome b5 by Ultraviolet Light
Fig. 1. Stationary absorption spectra of completely oxidized (black) and completely reduced (red) Cyt b5
Fig. 2. UV-Vis absorption spectra of Cyt b5 with/without irradiation at 280 nm
(a): Before illumination; (b): Upon illumination
Fig. 3. UV-Vis absorption spectra of chemical reduction for Cyt b5 with sodium dithionite
(a): The absorption spectra of chemical reduction for Cty b5 in soret band with sodium dithionite; (b): The obsorption spectra of chemical reduction for Cty b5 in Q band with sodium dithionite
Fig. 4. UV-Vis absorbance change of Cyt b5 irradiated by xenon lamp at different fixed wavelengths
(a): 254 nm; (b): 280 nm; (c): 412 nm; (d): 423 nm
Fig. 5. Curve of irradiation reduction peak area of Cyt b5 solution under different pH conditions at 280 nm
(a): The peak area changes at 412 nm; (b): The peak area changes at 556 nm
Fig. 6. UV-Vis absorbance change of Cyt b5 added GSH under pH 7.4
Fig. 7. UV-Vis absorbance change of Cyt b5 added Met under pH 7.4
Fig. 8. UV-Vis absorbance change of Cyt b5 added imidazole under pH 7.4
(a): The peak area changes at 412 nm; (b): Area changes at 556 nm
Fig. 9. Fluorescene spectra of Cyt b5 at different irradiated time at 280 nm Frome
Fig. 10. CD spectra of Cyt b5 after irradiation with xenon lamp at 280 nm From
Get Citation
Copy Citation Text
. Spectroscopic Study on the Mechanism of Photoduction of Cytochrome b5 by Ultraviolet Light[J]. Spectroscopy and Spectral Analysis, 2022, 42(3): 821
Category: Orginal Article
Received: Feb. 1, 2021
Accepted: Mar. 12, 2021
Published Online: Apr. 19, 2022
The Author Email: