Spectroscopy and Spectral Analysis, Volume. 42, Issue 3, 821(2022)

Spectroscopic Study on the Mechanism of Photoduction of Cytochrome b5 by Ultraviolet Light

Figures & Tables(10)
Stationary absorption spectra of completely oxidized (black) and completely reduced (red) Cyt b5
UV-Vis absorption spectra of Cyt b5 with/without irradiation at 280 nm(a): Before illumination; (b): Upon illumination ccyt b5=5×10-6 mol·L-1
UV-Vis absorption spectra of chemical reduction for Cyt b5 with sodium dithionite(a): The absorption spectra of chemical reduction for Cty b5 in soret band with sodium dithionite; (b): The obsorption spectra of chemical reduction for Cty b5 in Q band with sodium dithionite ccyt b5=3×10-6 mol·L-1, from a to e, cNa2S2O4=0, 3, 6, 9, 12×10-6 mol·L-1, respectively
UV-Vis absorbance change of Cyt b5 irradiated by xenon lamp at different fixed wavelengths(a): 254 nm; (b): 280 nm; (c): 412 nm; (d): 423 nm
Curve of irradiation reduction peak area of Cyt b5 solution under different pH conditions at 280 nm(a): The peak area changes at 412 nm; (b): The peak area changes at 556 nm
UV-Vis absorbance change of Cyt b5 added GSH under pH 7.4 a: Cyt b5; b: Cyt b5 irradiated at 25 min; c: Cyt b5-GSH irradiated at 25 min
UV-Vis absorbance change of Cyt b5 added Met under pH 7.4 a: Cyt b5; b: in dark 30 min; c: Cyt b5 irradiated at 5 min; d: Cyt b5-Met irradiated at 5 min
UV-Vis absorbance change of Cyt b5 added imidazole under pH 7.4(a): The peak area changes at 412 nm; (b): Area changes at 556 nm
Fluorescene spectra of Cyt b5 at different irradiated time at 280 nm Frome a to f, the irradiation times were 0, 5, 10, 15, 20, 25 min, respectively
CD spectra of Cyt b5 after irradiation with xenon lamp at 280 nm From a to c, the irradiation times were 0, 10, 25 min, respectively
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. Spectroscopic Study on the Mechanism of Photoduction of Cytochrome b5 by Ultraviolet Light[J]. Spectroscopy and Spectral Analysis, 2022, 42(3): 821

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Paper Information

Category: Orginal Article

Received: Feb. 1, 2021

Accepted: Mar. 12, 2021

Published Online: Apr. 19, 2022

The Author Email:

DOI:10.3964/j.issn.1000-0593(2022)03-0821-07

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