Acta Optica Sinica, Volume. 43, Issue 1, 0122001(2023)

Design of Off-Axis Three-Mirror System with Large Curved Field of View for Two-Photon Imaging of Cerebral Cortex of Mice

Jian Huang1, Zhenqiao Zhou2、*, Min Li2, and Hongbo Jia2
Author Affiliations
  • 1School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing , Jiangsu 210094, China
  • 2Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou , Jiangsu 215163, China
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    Figures & Tables(14)
    Cross-sectional structure of mouse brain
    Schematic diagram of coaxial triple reverse structure
    1/Rpet varying with β1 and β2
    Relationship between working distance behind system L3 and minimum radius of curvature Rpet of visual field
    Schematic diagram of structural restriction
    Structural diagram of optimized system
    System point diagram
    Circled energy distribution
    Full field aberration distribution
    Design drawing of dual objective lens combined use scheme
    • Table 1. Design parameters of optical system

      View table

      Table 1. Design parameters of optical system

      ParameterValue

      Wavelength /nm

      Field of view /[(°)×(°)]

      Radius of curvature of visual field /mm

      NA

      Matching field of view /mm

      920

      9×18

      6

      0.15

      3.5

    • Table 2. Initial structural parameters of three mirrors

      View table

      Table 2. Initial structural parameters of three mirrors

      MirrorRadius /mmDistance /mmConic

      M1

      M2

      -200.501

      -219.512

      -10

      10

      -468.089

      5153.146

      M3-12.149-60.069
    • Table 3. Parameters of each mirror after optimization

      View table

      Table 3. Parameters of each mirror after optimization

      MirrorRadius /mmDistance /mmConicx tilt /(°)
      M1-258.450-10910.06931.485

      M2

      M3

      -135.356

      -12.310

      10

      -6

      -421.540

      0.937

      -49.544

      24.615

    • Table 4. xy polynomial coefficients of free-form surfaces

      View table

      Table 4. xy polynomial coefficients of free-form surfaces

      ParameterM1M2M3
      y0.012-0.5771.146

      x2

      y2

      x2y

      y3

      x4

      x2y2

      y4

      x4y

      x2y3

      y5

      x6

      x4y2

      x2y4

      y6

      -136.212

      29.701

      161.257

      12.218

      1305.665

      1554.671

      -195.878

      1.848×104

      7187.777

      1.182×104

      -3.234×104

      7.098×105

      4.256×105

      1.372×105

      -331.288

      42.460

      -352.956

      327.263

      1.218×104

      -7030.459

      -5152.896

      -4.361×105

      -2.177×105

      2.049×104

      -1.302×107

      9.665×106

      -7.519×105

      9.774×105

      3.841

      26.914

      -712.502

      -712.529

      9232.172

      1.268×104

      4183.796

      -2.512×104

      -1.407×105

      -4.179×104

      1.458×106

      3.597×106

      1.857×106

      9.034×105

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    Jian Huang, Zhenqiao Zhou, Min Li, Hongbo Jia. Design of Off-Axis Three-Mirror System with Large Curved Field of View for Two-Photon Imaging of Cerebral Cortex of Mice[J]. Acta Optica Sinica, 2023, 43(1): 0122001

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    Paper Information

    Category: Optical Design and Fabrication

    Received: May. 19, 2022

    Accepted: Jun. 20, 2022

    Published Online: Jan. 6, 2023

    The Author Email: Zhou Zhenqiao (zhouzq@sibet.ac.cn)

    DOI:10.3788/AOS221159

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