Chinese Optics Letters, Volume. 17, Issue 8, 081701(2019)
Spectrophotometer with enhanced sensitivity for uric acid detection
Figures & Tables(9)
Fig. 2. Samples with different uric acid concentrations (a) before and (b) after stirring.
Fig. 3. Light beam at 10 mm distance from the input fiber. (a) Halogen beam. (b) Gray-scale percentage for halogen light. (c) LED beam (460 nm wavelength). (d) Gray-scale percentage for LED light.
Fig. 4. Spectrometer output spectra. (a) Halogen. (b) LED (460 nm wavelength).
Fig. 5. Light absorbance with variations of uric acid concentration at wavelengths of 460, 525, and 630 nm. (a) Halogen. (b) LED.
Fig. 6. Human urine sample in cuvette after mixing with NaOH solution.
Fig. 7. Absorbance analysis on human urine sample at 460 nm wavelength.
Table 1. Comparison of Light Characteristics Between Halogen and LED Light Sources at 460 nm Wavelength
View tableView in ArticleTable 1. Comparison of Light Characteristics Between Halogen and LED Light Sources at 460 nm Wavelength
Parameter Halogen LED (μW) 15.4 1.7 ( ) 33.18 9.08 (nlm) 7.3337 2.9582 (mlm/W) 0.4762 1.7401
Table 2. Comparison of Spectrophotometer Performance
View tableView in ArticleTable 2. Comparison of Spectrophotometer Performance
Light Source Wavelength (nm) Sensitivity (dL/mg) Accuracy (%) Halogen 460 0.0012 86.51 525 0.0011 91.17 630 0.0008 84.27 LED 460 0.0046 96.62 525 0.0031 77.57 630 0.0045 79.43
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Nurfatihah Che Abd Rashid, Nor Hafizah Ngajikin, Asrul Izam Azmi, Rashidah Arsat, Suhaila Isaak, Noran Azizan Cholan, Nur Ellina Azmi. Spectrophotometer with enhanced sensitivity for uric acid detection[J]. Chinese Optics Letters, 2019, 17(8): 081701
Paper Information
Category: Medical optics and biotechnology
Received: Jan. 7, 2019
Accepted: May. 6, 2019
Posted: May. 14, 2019
Published Online: Jul. 26, 2019
The Author Email: Nor Hafizah Ngajikin (norhafizah@uthm.edu.my)
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