Optics and Precision Engineering, Volume. 32, Issue 16, 2455(2024)

Design of gene sequencing fluorescence microscopic system with dual-band LED illumination

Meichen LIU1...2, Qingyu MENG1,*, Hongwen ZHANG1, Kejun WANG1, Yunhui LI1, Siqi WANG1,2, and Jiangnan WANG12 |Show fewer author(s)
Author Affiliations
  • 1Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences, Changchun30033,China
  • 2University of Chinese Academy of Sciences,Beijing100049,China
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    Figures & Tables(13)
    Optical path of dual-band gene sequencing fluorescence microscopic imaging system
    Relation spectral power distribution of R-LED and G-LED and transmission of filter at different wavelengths
    Layout of the illuminating optical system
    Schematic of the illumination system simulation
    Layout of the imaging optical system
    Structure of radiator
    Structure of the optical filters
    Structure of radiator
    Complete machine system
    Imaging test
    Result of testing
    Schematic diagram of sampling
    • Table 1. Specification of the optical system

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      Table 1. Specification of the optical system

      ParameterSpecifications
      Resolution/pixel5 472×3 648
      Pixel size/μm22.4×2.4
      Excitation wavelength/nm525, 620
      Fluorescence wavelength/nm570 ,680
      Imaging range/mm1.31×0.88
      Systemic resolution/μm≤2
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    Meichen LIU, Qingyu MENG, Hongwen ZHANG, Kejun WANG, Yunhui LI, Siqi WANG, Jiangnan WANG. Design of gene sequencing fluorescence microscopic system with dual-band LED illumination[J]. Optics and Precision Engineering, 2024, 32(16): 2455

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    Paper Information

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    Received: Feb. 29, 2024

    Accepted: --

    Published Online: Nov. 18, 2024

    The Author Email: MENG Qingyu (mengqy@ciomp.ac.cn)

    DOI:10.37188/OPE.20243216.2455

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