Acta Optica Sinica, Volume. 39, Issue 8, 0830002(2019)

Structural Design and Performance Analysis of Specific Protein Analyzer Based on Concave Grating

Junwei Li1, Yi Ni1、*, Yu Guo1, Xiaotian Zhang1, Daohong Zhong1, and Junxing Fan2
Author Affiliations
  • 1 School of Internet of Things Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China
  • 2 Wuxi Cross Medical Co., Ltd., Wuxi, Jiangsu 214028, China
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    Figures & Tables(16)
    Post-spectral technology
    Grating diffraction principle. (a) Holographic concave grating; (b) Rowland grating
    3D principle schematic. (a) Spectroscopic structure; (b) overall light path
    Spot diagrams. (a) Spot diagram at 340 nm; (b) spot diagram at 405 nm; (c) spot diagram at 505 nm; (d) spot diagram at 600 nm; (e) spot diagram at 660 nm; (f) spot diagram at 790 nm
    RMS of Y' versus wavelength
    Prototype structure. (a) Schematic of spectrophotometer structure; (b) photograph
    Actual spectrogram
    Schematic of current-voltage amplification principle
    Repeatability test. (a) Repeatability at 350 nm; (b) repeatability at 475 nm; (c) repeatability at 800 nm
    Fitted lines. (a) Fitted line at 340 nm of #1; (b) fitted line at 405 nm of #1; (c) fitted line at 340 nm of #2; (d) fitted line at 405 nm of #2
    Relationship between absolute value of relative bias and relative concentration
    • Table 1. Main technical parameters of optical path

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      Table 1. Main technical parameters of optical path

      ParameterValue
      Spectral range /nm320-800
      Grating density /(g·mm-1)950
      Radius of grating /mm49.8
      Diffraction order-1
      Radius of lens 1 (7) /mm80.0
      Radius of lens 2 (8) /mm60.1
      Angle between lens 1 and lens 2 /(°)10.4
      Distance from slit to grating center /mm39.2
    • Table 2. Accuracy of two devices

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      Table 2. Accuracy of two devices

      ItemMeasured absorbance /arb. units
      #1#2
      Max0.98650.49750.98050.4972
      Min0.98610.49650.97690.4942
      Average0.98620.49700.97800.4955
      Accuracy-0.0198-0.0110-0.0280-0.0125
    • Table 3. Stability of two devices

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      Table 3. Stability of two devices

      ItemMeasured absorbance /arb. units
      K2Cr2O7(340 nm)CuSO4 (660 nm)
      #1#2#1#2
      Max0.49550.49540.51700.5207
      Min0.49410.49060.51430.5176
      Average0.49480.49350.51560.5192
      Stability0.00140.00480.00270.0031
    • Table 4. Relative bias parameters of two devices

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      Table 4. Relative bias parameters of two devices

      Relative concentrationAverage absorbance /arb. unitsDn/%
      K2Cr2O7C6H5NO3K2Cr2O7C6H5NO3
      #1#2#1#2#1#2#1#2
      41.41301.44731.49381.4574-0.324-0.243-0.515-0.601
      51.77571.78971.85511.80740.236-1.287-1.102-1.342
      62.12342.13412.22272.1462-0.092-1.888-1.217-2.346
      72.46942.45432.56372.4941-0.399-3.275-2.314-2.713
      82.79812.80292.91642.8282-1.237-3.335-2.745-3.453
      93.12613.09763.25943.1206-1.915-5.034-3.368-5.300
      103.44523.44133.60843.4537-2.704-5.041-3.707-5.662
    • Table 5. Comparison of instrument performance

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      Table 5. Comparison of instrument performance

      Model721UV-1800DR39007100 VIS#1
      Optical structure (elements)Grating & mirrorC-TConcave grating & filterLED & monochromatorRowland Grating & lens
      Spectral range /nm340-1000200-1100320-1100320-1100320-800
      Wavelength repeatability /nm1.00.30.10.50.5
      Wavelength accuracy /nm2.00.51.51.01.0
      Absorbance accuracy /arb. units-0.0040.005-0.020.003-0.010.01-0.02
      Absorbance stability /arb. units0.002--0.0020.001
      Absorbance range /arb. units-0.301-3.0-0.301-3.00-3.00-3.30-3.6
      Place of originShanghaiBeijingUSAGermany-
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    Junwei Li, Yi Ni, Yu Guo, Xiaotian Zhang, Daohong Zhong, Junxing Fan. Structural Design and Performance Analysis of Specific Protein Analyzer Based on Concave Grating[J]. Acta Optica Sinica, 2019, 39(8): 0830002

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    Paper Information

    Category: Spectroscopy

    Received: Mar. 13, 2019

    Accepted: Apr. 23, 2019

    Published Online: Aug. 7, 2019

    The Author Email: Ni Yi (niy2011@163.com)

    DOI:10.3788/AOS201939.0830002

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