Acta Physica Sinica, Volume. 69, Issue 16, 168703-1(2020)

Phasor analysis of fluorescence lifetime data and its application

Dan-Ying Lin*... Jing-Jing Niu, Xiong-Bo Liu, Xiao Zhang, Jiao Zhang, Bin Yu and Jun-Le Qu* |Show fewer author(s)
Author Affiliations
  • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
  • show less
    Figures & Tables(8)
    Schematic diagram of fluorescence lifetime measurement and phasor analysis (PA):(a) Frequency domain method; (b) lifetime phasor of single-exponential decay; (c) lifetime phasor of bi-exponential decay; (d) time-correlated single photon counting (TCSPC) method.
    Schematic diagram of phasor-FLIM application:(a) Fluorescence intensity image with untreated lifetime information; (b) lifetime phasor plot obtained by PA analysis; (c) direct analysis of lifetime phasors; (d) phasor-mapped FLIM image based on phasor clustering analysis and pseudo-color assignment.
    Application classification diagram of phasor-FLIM.
    Phasor-FLIM was used to analyze the change of NADH/NAD+ ratio under the stimulation of hypoxia and mitochondrial toxic drug potassium cyanide, for studying the change of metabolic state of cells[27].
    Phasor-FLIM was used in quantitative FRET efficiency detection of a RhoA-kRas single chain biosensor, studying interaction between proteins[28].
    Phasor-FLIM was used to analyze the changes of pH value of cells in normal state (at rest) and under oxidative stress[32].
    Phasor-FLIM clustering analysis and pseudo-color assignment was used to enhance visualization of pathological features of basal cell carcinoma (BCC) sections stained with H&E, assisting pathological diagnosis[36].
    Phasor-FLIM cluster analysis was used to filter out the photons in the annular depletion region in stimulated radiation depletion (STED) imaging, improving the resolution of super-resolution imaging[39].
    Tools

    Get Citation

    Copy Citation Text

    Dan-Ying Lin, Jing-Jing Niu, Xiong-Bo Liu, Xiao Zhang, Jiao Zhang, Bin Yu, Jun-Le Qu. Phasor analysis of fluorescence lifetime data and its application[J]. Acta Physica Sinica, 2020, 69(16): 168703-1

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category:

    Received: Apr. 15, 2020

    Accepted: --

    Published Online: Jan. 4, 2021

    The Author Email: Qu Jun-Le (jlqu@szu.edu.cn)

    DOI:10.7498/aps.69.20200554

    Topics