Acta Physica Sinica, Volume. 69, Issue 16, 168703-1(2020)
Fig. 1. Schematic diagram of fluorescence lifetime measurement and phasor analysis (PA):(a) Frequency domain method; (b) lifetime phasor of single-exponential decay; (c) lifetime phasor of bi-exponential decay; (d) time-correlated single photon counting (TCSPC) method.
Fig. 2. Schematic diagram of phasor-FLIM application:(a) Fluorescence intensity image with untreated lifetime information; (b) lifetime phasor plot obtained by PA analysis; (c) direct analysis of lifetime phasors; (d) phasor-mapped FLIM image based on phasor clustering analysis and pseudo-color assignment.
Fig. 4. Phasor-FLIM was used to analyze the change of NADH/NAD+ ratio under the stimulation of hypoxia and mitochondrial toxic drug potassium cyanide, for studying the change of metabolic state of cells[27].
Fig. 5. Phasor-FLIM was used in quantitative FRET efficiency detection of a RhoA-kRas single chain biosensor, studying interaction between proteins[28].
Fig. 6. Phasor-FLIM was used to analyze the changes of pH value of cells in normal state (at rest) and under oxidative stress[32].
Fig. 7. Phasor-FLIM clustering analysis and pseudo-color assignment was used to enhance visualization of pathological features of basal cell carcinoma (BCC) sections stained with H&E, assisting pathological diagnosis[36].
Fig. 8. Phasor-FLIM cluster analysis was used to filter out the photons in the annular depletion region in stimulated radiation depletion (STED) imaging, improving the resolution of super-resolution imaging[39].
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Dan-Ying Lin, Jing-Jing Niu, Xiong-Bo Liu, Xiao Zhang, Jiao Zhang, Bin Yu, Jun-Le Qu.
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Received: Apr. 15, 2020
Accepted: --
Published Online: Jan. 4, 2021
The Author Email: Qu Jun-Le (jlqu@szu.edu.cn)