Journal of Innovative Optical Health Sciences, Volume. 17, Issue 1, 2330011(2024)
Single-cell manipulation by two-dimensional micropatterning
Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape, cytoskeleton and organelle, as well as the nucleus morphology and genetic expression. The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis, cellular standardization, and in vivo environment mimicking. Here, we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques, including photolithographic micropatterning and soft lithography micropatterning. Moreover, we summarize the application of micropatterning technique in controlling cytoskeleton, cell migration, nucleus and gene expression, as well as intercellular communication.
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Xuehe Ma, Haimei Zhang, Shiyu Deng, Qiushuo Sun, Qingsong Hu, Yuhang Pan, Fen Hu, Imshik Lee, Fulin Xing, Leiting Pan. Single-cell manipulation by two-dimensional micropatterning[J]. Journal of Innovative Optical Health Sciences, 2024, 17(1): 2330011
Category: Research Articles
Received: Aug. 26, 2023
Accepted: Nov. 29, 2023
Published Online: Feb. 28, 2024
The Author Email: Xing Fulin (xingfulin@nankai.edu.cn), Pan Leiting (plt@nankai.edu.cn)