Laser & Optoelectronics Progress, Volume. 61, Issue 6, 0618015(2024)
Three-Dimensional Orientation and Localization Microscopy of Single Molecules Using Super-Resolution Imaging Technology (Invited)
Super-resolution microscopy with nanoscale spatial resolution has become an important imaging tool in life science research. As a super-resolution technique, single-molecule localization microscopy enables us to localize, identify, and study the unique behaviors of single molecules. At the single-molecule level, the emitted fluorescence signal is highly anisotropic. Resolving the polarization or three-dimensional orientation of single fluorescent molecules is an emerging field in super-resolution microscopy. In this review, we describe the three-dimensional orientation of single molecules through super-resolution imaging techniques. These techniques include fluorescence polarization microscopy and single-molecule orientation imaging through point spread function engineering. Furthermore, we discuss other polarization super-resolution imaging approaches for the applications of live cells and single nanoparticle studies. Finally, we discuss the potential challenges and future research needs of single-molecule orientation localization microscopy. These challenges and requirements can provide in-depth insights into future research in life sciences.
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Ruihang Zhao, Jin Lu. Three-Dimensional Orientation and Localization Microscopy of Single Molecules Using Super-Resolution Imaging Technology (Invited)[J]. Laser & Optoelectronics Progress, 2024, 61(6): 0618015
Category: Microscopy
Received: Dec. 22, 2023
Accepted: Feb. 18, 2024
Published Online: Mar. 20, 2024
The Author Email: Lu Jin (luj@nanoctr.cn)