Identification of Algae Community Discrete Three-Dimensional Fluorescence Spectrum Based on SWTATLD

Zhao Cheng; Nanjing Zhao; Gaofang Yin; Xiaoling Zhang; Jianguo Li; Wenqing Liu

doi:10.3788/AOS202141.1430001

Acta Optica Sinica, Volume. 41, Issue 14, 1430001(2021)

Identification of Algae Community Discrete Three-Dimensional Fluorescence Spectrum Based on SWTATLD

Zhao Cheng^1,2,3, Nanjing Zhao^1,3、aff***, Gaofang Yin^1,3, Xiaoling Zhang⁴, Jianguo Li^1,3, and Wenqing Liu^1,3

¹Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China

²University of Science and Technology of China, Hefei, Anhui 230026, China

³Key Laboratory of Environmental Optical Monitoring Technology of Anhui Province, Hefei, Anhui 230031, China

⁴Anhui University, Hefei, Anhui 230601, China

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Figures & Tables(7)

Fig. 1. Fluorescence spectra of each alga. (a)－(e) Three－dimensional fluorescence spectra；(f)－(j) discrete three－dimensional fluorescence spectra

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Fig. 2. Spectrum profiles of algal mixtures by PARAFAC. (a) Excitation spectra; (b) emission spectra

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Fig. 3. Spectrum profiles of algal mixtures by SWTATLD. (a) Excitation spectra; (b) emission spectra

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Fig. 4. Contrastive analysis between PARAFAC and SWTATLD results

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Table 1. Concentration of each alga sample

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Table 1. Concentration of each alga sample

Sample No.	Mass concentration /(μg·L^－1)
Sample No.	Microcystis aeruginosa	Scenedesmus obliquus	Nitzschia sp.	Peridinium umbonatum var. inaequale	Cryptomonas obovata.
1	77.16	26.08	55.61	70.66	125.77
2	36.69	14.78	26.42	32.75	64.52
3	21.67	6.93	14.95	27.91	39.46
4	14.98	3.91	9.11	6.92	25.05
5	7.95	2.68	5.08	45.65	12.95
6	58.42	0.80	44.81	21.58	1.36
7	29.78	49.57	21.26	7.65	9.09
8	17.56	25.61	13.51	36.85	27.85
9	11.84	14.82	7.74	63.29	40.13
10	8.20	9.25	4.12	45.9	25.67
11	27.33	4.54	8.80	2.40	13.21
12	12.81	58.41	3.31	4.41	25.09
13	7.23	27.90	1.45	20.18	9.89
14	4.86	16.60	18.12	9.67	2.67
15	30.12	10.95	66.02	34.50	7.51

Table 2. Prediction results of algal mixture samples by PARAFAC

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Table 2. Prediction results of algal mixture samples by PARAFAC

No.	Microcystis aeruginosa		Scenedesmus obliquus		Nitzschia sp.		Peridinium umbonatum var. inaequale		Cryptomonas obovata.
No.	C /(μg·L^－1)	R /%	C /(μg·L^－1)	R /%	C /(μg·L^－1)	R /%	C /(μg·L^－1)	R /%	C /(μg·L^－1)	R /%
1	65.04	84.29	29.27	112.23	46.63	83.85	81.25	114.99	103.46	82.26
2	42.78	116.59	13.10	88.63	30.79	116.54	38.63	117.95	51.24	79.42
3	18.63	85.97	7.52	108.51	11.26	75.32	34.36	123.11	32.53	82.44
4	12.92	86.25	4.74	121.22	7.45	81.78	7.29	105.35	25.68	102.52
5	7.03	88.43	2.64	98.51	4.28	84.25	53.36	116.89	9.59	74.05
6	48.01	82.18	0.89	111.25	36.85	82.24	20.23	93.74	1.41	103.68
7	25.87	86.87	47.70	96.23	18.4	86.55	7.57	98.95	7.18	78.99
8	13.79	78.53	28.31	110.54	15.32	113.40	41.48	112.56	22.89	82.19
9	13.12	110.81	13.68	92.31	8.42	108.79	77.31	122.15	48.50	120.86
10	7.03	85.73	10.91	117.95	3.52	85.44	40.62	88.50	22.69	88.39
11	31.40	114.89	5.07	111.67	8.93	101.48	2.14	89.17	10.26	77.67
12	12.37	96.57	50.78	86.94	2.74	82.78	4.17	94.56	25.87	103.11
13	8.22	113.69	34.09	122.19	1.13	77.93	26.35	130.57	7.14	72.19
14	3.85	79.22	15.89	95.72	14.79	81.62	11.62	120.17	2.43	91.01
15	24.35	80.84	11.91	108.77	50.67	76.75	39.18	113.56	6.96	92.68
R_A/%	92.73±13.99		105.51±11.58		89.25±13.68		109.48±13.47		88.76±13.60

Table 3. Prediction results of algal mixture samples by SWTATLD

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Table 3. Prediction results of algal mixture samples by SWTATLD

No.	Microcystis aeruginosa		Scenedesmus obliquus		Nitzschia sp.		Peridinium umbonatum var. inaequale		Cryptomonas obovata.
No.	C /(μg·L^－1)	R /%	C /(μg·L^－1)	R /%	C /(μg·L^－1)	R /%	C /(μg·L^－1)	R /%	C /(μg·L^－1)	R /%
1	75.34	97.64	24.37	93.44	56.33	101.29	67.24	95.16	134.46	106.91
2	34.25	93.34	13.41	90.73	24.59	93.07	31.36	95.76	62.93	97.54
3	19.72	91.00	7.04	101.59	15.36	102.74	28.49	102.08	41.35	104.79
4	15.06	100.53	3.87	98.98	9.01	98.90	6.83	98.69	27.64	110.34
5	7.83	98.49	2.74	102.24	5.26	103.54	42.74	93.63	13.41	103.55
6	54.28	92.91	0.76	95.00	47.2	105.33	19.72	91.38	1.45	106.62
7	28.75	96.54	45.82	92.43	20.41	96.00	7.76	101.44	8.96	98.57
8	16.29	92.77	27.15	106.01	14.64	108.36	33.58	91.13	28.63	102.80
9	12.16	102.70	14.42	97.30	7.92	102.32	59.78	94.45	43.59	108.62
10	8.34	101.71	9.53	103.02	4.27	103.64	46.35	100.98	24.83	96.73
11	25.42	93.01	4.38	96.48	8.94	101.59	2.33	97.08	14.57	110.29
12	12.04	93.99	55.72	95.39	3.35	101.21	4.47	101.36	25.82	102.91
13	7.12	98.48	26.8	96.06	1.52	104.83	20.98	103.96	10.03	101.42
14	4.99	102.67	16.37	98.61	17.68	97.57	9.53	98.55	2.71	101.50
15	28.53	94.72	11.36	103.74	69.45	105.19	32.15	93.19	7.58	100.93
R_A /%	96.70±3.94		98.07±4.48		101.71±3.97		97.26±4.11		103.57±4.34

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Zhao Cheng, Nanjing Zhao, Gaofang Yin, Xiaoling Zhang, Jianguo Li, Wenqing Liu. Identification of Algae Community Discrete Three-Dimensional Fluorescence Spectrum Based on SWTATLD[J]. Acta Optica Sinica, 2021, 41(14): 1430001

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Paper Information

Category: Spectroscopy

Received: Dec. 29, 2020

Accepted: Feb. 5, 2021

Published Online: Jul. 11, 2021

The Author Email: Zhao Nanjing (njzhao@aiofm.ac.an)

DOI:10.3788/AOS202141.1430001

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