Conditions for Stably Detecting Comprehensive Toxicity of Water by Algal Photosynthesis Inhibition Method

Peilong Qi; Gaofang Yin; Tingting Gan; Nanjing Zhao; Mengyuan Gu; Min Chen; Zhichao Ding; Renqing Jia; Jinjing Liu; Mingjun Ma; Ruifang Yang; Li Fang

doi:10.3788/AOS202242.1217001

Acta Optica Sinica, Volume. 42, Issue 12, 1217001(2022)

Conditions for Stably Detecting Comprehensive Toxicity of Water by Algal Photosynthesis Inhibition Method

Peilong Qi^1,2,3, Gaofang Yin^2,3、*, Tingting Gan^1,2,3、**, Nanjing Zhao^2,3, Mengyuan Gu^1,2,3, Min Chen^1,2,3, Zhichao Ding^1,2,3, Renqing Jia^1,2,3, Jinjing Liu^1,2,3, Mingjun Ma^1,2,3, Ruifang Yang^2,3, and Li Fang^2,3

Author Affiliations

¹School of Environment Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230026, Anhui, China

²Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, Anhui, China

³Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei 230031, Anhui, China

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Figures & Tables(8)

Fig. 1. Inhibition ratios of four photosynthetic fluorescence parameters of Chlorella pyrenoidosa with different mass concentrations under stress by DCMU for 1 h. (a) α; (b) Fv/Fm; (c) Yield; (d) rP

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Fig. 2. Fv/Fm of blank control group for different mixing volume ratios. (a) Mixed samples to be tested with same algae concentration; (b) tested algae samples with same concentration before mixing

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Fig. 3. Inhibition rates of four photosynthetic fluorescence parameters of Chlorella pyrenoidosa with same mass

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Fig. 4. Inhibition ratios of four photosynthetic fluorescence parameters of Chlorella pyrenoidosa with different mass

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Fig. 5. Fv/Fm of Chlorella pyrenoidosa varying with time under different temperatures

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Fig. 6. Relative change rate of inhibition rate of four photosynthetic fluorescence parameters for Chlorella pyrenoidosa under DCMU stress for 5 min at different temperature compared with that at 20 ℃. (a) α; (b) Fv/Fm; (c) Yield; (d) rP

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Table 1. Parameters acquired by rapid light curve (RLC)^[16]

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Table 1. Parameters acquired by rapid light curve (RLC)^[16]

Parameter	Designation	Physiological significance
α	Maximum light utilization coefficient or initialslope of rapid light curve	Capturing ability of photosyntheticsystem for light energy
Fv/Fm	Maximum photochemicalquantum efficiency of PS II	Characterization of photosyntheticsystem PS II activity
Yield	Actual quantum yield of PS II under light	Photosynthetic rate
rP	Relative photosynthetic electron transfer rate	Photosynthetic rate, which corresponds to therelease of O₂ and the fixed rate of CO₂

Table 2. Variation of photosynthetic activity parameters for different mass concentrations (C) of Chlorella pyrenoidosa under stress of different mass concentrations of DCMU for 1 h

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Table 2. Variation of photosynthetic activity parameters for different mass concentrations (C) of Chlorella pyrenoidosa under stress of different mass concentrations of DCMU for 1 h

Mass concentrationof DCMU /(μg·L^-1)	Parameter	C=100 μg·L^-1	C=200 μg·L^-1	C=400 μg·L^-1	C=600 μg·L^-1
	α	0.5910±0.0078	0.5940±0.0113	0.6060±0.0078	0.6100±0.0085
0	Fv/Fm	0.6320	0.6330±0.0035	0.6360±0.0035	0.6360±0.0028
	rP	60.7150±1.4354	61.2250±1.5627	61.7200±0.8061	60.7300±0.3394
	Yield	0.4380±0.0071	0.4550±0.0120	0.4560±0.0177	0.4320±0.0014
	α	0.4160±0.0156	0.4250±0.0113	0.4180±0.0254	0.3970±0.0035
5	Fv/Fm	0.5010	0.4920±0.0100	0.4900±0.0014	0.4950±0.0028
	rP	41.0790±1.3562	42.9050±0.5020	43.9100±0.3536^*	40.2300±0.4525
	Yield	0.2880±0.0144	0.3060±0.0028	0.3030±0.0028	0.2910±0.0029
	α	0.2870±0.0054	0.3070±0.0057^*	0.2790±0.0085	0.2720±0.0028
10	Fv/Fm	0.3990±0.0064	0.3970±0.0184	0.3910±0.0028	0.3900±0.0042
	rP	31.9140±2.0739	32.8350±0.7707	32.8200±0.5940	29.1550±0.5440
	Yield	0.2270±0.0080	0.2305±0.0110	0.2390±0.0028	0.2130±0.0057^*
	α	0.2190±0.0175	0.2310±0.0120	0.2270±0.0057	0.2090±0.0127
20	Fv/Fm	0.3620±0.0042	0.3570±0.0163	0.3530	0.3480±0.0035
	rP	28.4050±0.8132	30.5850±0.4738^*	26.4200±0.5374	27.9600±1.8243
	Yield	0.2060±0.0049	0.2190±0.0078	0.1910±0.0070^*	0.1940±0.0049
	α	0.1630±0.0062	0.1600±0.0069	0.1800±0.0035^*	0.1550±0.0057
40	Fv/Fm	0.2900±0.0057	0.2640±0.0014^*	0.2740±0.0127	0.2680±0.0021^*
	rP	19.3190±0.1281	20.0050±0.6010	20.7000±0.3677^*	18.4150±0.1626
	Yield	0.1420±0.0016	0.1360±0.0049	0.1510±0.0021^*	0.1290±0.0007^*
	α	0.1070	0.0960±0.0057	0.0910±0.0021^*	0.0950±0.0100
80	Fv/Fm	0.2200±0.0113	0.2280±0.0057	0.2180±0.0035	0.2210±0.0007
	rP	12.6600±0.1000	11.0650±0.8270	9.6610±0.9185^*	10.7590±1.4722
	Yield	0.0890±0.0021	0.0860±0.0014	0.0820±0.0141	0.0880

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Peilong Qi, Gaofang Yin, Tingting Gan, Nanjing Zhao, Mengyuan Gu, Min Chen, Zhichao Ding, Renqing Jia, Jinjing Liu, Mingjun Ma, Ruifang Yang, Li Fang. Conditions for Stably Detecting Comprehensive Toxicity of Water by Algal Photosynthesis Inhibition Method[J]. Acta Optica Sinica, 2022, 42(12): 1217001

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