In recent years, methods for large-depth optical imaging of biological tissues, such as optical coherence tomography, multi-photon imaging, and adaptive optics, have been developed continuously. This paper outlines a series of important advances in large-depth quantitative optical imaging of biological tissues achieved by the College of Optical Science and Engineering of Zhejiang University in recent years, including structural and functional optical coherence tomography, large-depth cerebral vascular imaging based on three-photon fluorescence microscopy, and new wavefront correction methods for distortion errors. It also summarizes the ways of quantitatively characterizing the optical images obtained by these methods to acquire physiological and pathological information about biological tissues.