Laser & Optoelectronics Progress, Volume. 61, Issue 6, 0618001(2024)

Recent Advances in Super-Resolution and Optical Sectioning of Digital-Micromirror Device-Based Structured-Illumination Microscopy (Invited)

Wang Ma1,2, Jia Qian1, Siying Wang1, Rui Ma1, Xing Li1, Shipei Dang1, Xing Li1, Chen Bai1,2, Dan Dan1,2、*, and Baoli Yao1,2、**
Author Affiliations
  • 1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, Shaanxi, China
  • 2University of Chinese Academy of Sciences, Beijing 100049, China
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    Figures & Tables(24)
    The principle of spectrum expansion in SIM. (a) The overlapping of the sample and the illumination patterns produces low-frequency Moiré fringes through frequency beating; (b) the expansion and fusion of the sample spectrum under structured illumination along one single orientation; (c) the isotropic expansion and fusion of the sample spectrum under structured illumination along three orientations
    The principle and flowchart of spatial domain reconstruction of SR-SIM image[23]. (a) Schematic diagram of the PSF compression in SDR; (b) the PSF's FWHM value variation with the spatial frequency of the illumination fringes; (c) the comparison of SDR and FDR workflow
    Principle of the super-resolution and optical section integrated SIM[41]
    Principle of DMD. (a) DMD chip; (b) structure of the DMD micromirror array; (c) illustration of the incident and output light on the DMD; (d) schematic of the incident light modulation in three different states of micromirrors
    Schematic diagrams of generating structured illumination using DMD projection. (a) Grating projection method; (b) DMD projection method; (c) the conversion from binary periodic square patterns to sinusoidal patterns
    Typical optical paths for generating structured illumination based on SLM laser interferometry and DMD laser interferometry. (a) SLM[44]; (b) DMD[76]
    The model of DMD two-dimensional blazed diffractive gratings and the analysis of diffracted beams[98]
    The non-paraxial diffraction model of DMD[99]
    Timeline of DMD-SIM development
    The setup of LiDMD-SIM[102]
    Schematic of the hybrid multifocal structured illumination microscope[103]
    The dual color DMD-SIM microscope[104]
    The schematic of three-wavelength DMD-SIM super-resolution microscopy[105]
    The schematic of laser illumination speckle-free DMD-SIM super-resolution microscopy[106]
    Imaging results of DMD-3DSIM[76]
    The schematic of multi-color DMD-SIM based on grating dispersion compensation[107]
    Full-Color optical sectioning three-dimensional microscopy based on DMD-SIM[108]
    Schematic diagram of the C-SIM system[109]
    Imaging results of HDR-C-SIM[111]
    Fast optical sectioning algorithm of SIM based on Hilbert transform[113]
    Configuration of SIMscope3D and its imaging results[7]
    Imaging results of Fourier Domain-based High-Quality SIM optical sectioning reconstruction method[114]
    • Table 1. Comparison of different reconstruction algorithms for SR-SIM

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      Table 1. Comparison of different reconstruction algorithms for SR-SIM

      AlgorithmReconstruction domainReconstruction speedReconstruction qualityFeature
      Open-SIM59FDRSlowMiddleEasy to use
      Fair-SIM60FDRSlowLowImageJ plugin
      HiFi-SIM61FDRSlowHighHigh quality
      Hessian-SIM64FDRSlowMiddleFast dynamic imaging
      SDR-SIM23SDRFastHighNo Frequency Domain processing
      EFSD-SIM71SDRFastMiddleEstimation-free
      JSFR-SIM69SDR+FDRFastHighBackground suppression
      Direct-SIM68SDR+FDRFastMiddleEstimation-free
    • Table 2. Comparison of DMD-SIM with other types of SIM

      View table

      Table 2. Comparison of DMD-SIM with other types of SIM

      SIM techniqueSI generationImaging speedImaging qualityFeature
      Projection DMD-SIM40ProjectionFastExcellentCompact and low-cost
      Interference DMD-SIM76-77InterferenceFastExcellentSpecific beam incident angle
      Grating SIM19Projection/interferenceSlowModerateLow mechanical control
      SLM-SIM45-46InterferenceFastExcellentComplex polarization control
      Speckle SIM78-81Speckle illuminationSlowModerateLow speed
      TIRF SIM83-85Evanescent wave InterferenceSlowgoodTIRF mode required
      Photonic-chip SIM82Evanescent wave InterferenceMiddlegoodNo illumination objective lens
      Plasmonic SIM86-88SP wave InterferenceMiddlegoodPlasmon required
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    Wang Ma, Jia Qian, Siying Wang, Rui Ma, Xing Li, Shipei Dang, Xing Li, Chen Bai, Dan Dan, Baoli Yao. Recent Advances in Super-Resolution and Optical Sectioning of Digital-Micromirror Device-Based Structured-Illumination Microscopy (Invited)[J]. Laser & Optoelectronics Progress, 2024, 61(6): 0618001

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    Paper Information

    Category: Microscopy

    Received: Nov. 3, 2023

    Accepted: Dec. 2, 2023

    Published Online: Mar. 22, 2024

    The Author Email: Dan Dan (dandan@opt.ac.cn), Yao Baoli (yaobl@opt.ac.cn)

    DOI:10.3788/LOP240550

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