Journal of Innovative Optical Health Sciences, Volume. 18, Issue 1, 2450018(2025)

A 3D semantic segmentation network for accurate neuronal soma segmentation

Li Ma1,*... Qi Zhong1,**, Yezi Wang1,***, Xiaoquan Yang2,**** and Qian Du3,***** |Show fewer author(s)
Author Affiliations
  • 1School of Mechanical Engineering and Electronic Information, China University of Geosciences, Wuhan 430074, P. R. China
  • 2Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan 430074, P. R. China
  • 3Department of Electrical and Computer Engineering, Mississippi State University, Mississippi State, MS 39762, USA
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    Neuronal soma segmentation plays a crucial role in neuroscience applications. However, the fine structure, such as boundaries, small-volume neuronal somata and fibers, are commonly present in cell images, which pose a challenge for accurate segmentation. In this paper, we propose a 3D semantic segmentation network for neuronal soma segmentation to address this issue. Using an encoding-decoding structure, we introduce a Multi-Scale feature extraction and Adaptive Weighting fusion module (MSAW) after each encoding block. The MSAW module can not only emphasize the fine structures via an upsampling strategy, but also provide pixel-wise weights to measure the importance of the multi-scale features. Additionally, a dynamic convolution instead of normal convolution is employed to better adapt the network to input data with different distributions. The proposed MSAW-based semantic segmentation network (MSAW-Net) was evaluated on three neuronal soma images from mouse brain and one neuronal soma image from macaque brain, demonstrating the efficiency of the proposed method. It achieved an F1 score of 91.8% on Fezf2-2A-CreER dataset, 97.1% on LSL-H2B-GFP dataset, 82.8% on Thy1-EGFP-Mline dataset, and 86.9% on macaque dataset, achieving improvements over the 3D U-Net model by 3.1%, 3.3%, 3.9%, and 2.3%, respectively.

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    Li Ma, Qi Zhong, Yezi Wang, Xiaoquan Yang, Qian Du. A 3D semantic segmentation network for accurate neuronal soma segmentation[J]. Journal of Innovative Optical Health Sciences, 2025, 18(1): 2450018

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    Paper Information

    Category: Research Articles

    Received: May. 7, 2024

    Accepted: Jul. 10, 2024

    Published Online: Feb. 21, 2025

    The Author Email: Ma Li (mali@cug.edu.cn), Zhong Qi (zq20171001535@cug.edu.cn), Wang Yezi (cugwyz20000720@163.com), Yang Xiaoquan (xqyang@mail.hust.edu.cn), Du Qian (du@ece.msstate.edu)

    DOI:10.1142/S1793545824500184

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