Fig. 1. Optical system for 2P STED composite microscope

Fig. 2. Side view of optical layout in the microscope

Fig. 3. Point spread function scanning imaging in XY plane

Fig. 4. 2P imaging of fluorescent microspheres with different depth

Fig. 5. Resolution measurement by fluorescence microsphere imaging

Fig. 6. 2P STED composite microscope prototype

Fig. 7. Fluorescence in-situ hybridization (FISH) imaging

Fig. 8. 2P imaging of brain.

Fig. 9. Second harmonic imaging of skin.

Fig. 10. 2P depth imaging

Fig. 11. Super resolution imaging comparison of fluorescent microsphere