Two-photon (2P) stimulated emission depletion (STED) composite microscope has the potential to be widely used in clinical diagnosis of neurological diseases and brain science research. In this paper, based on the research of multi wavelengths selection, multi beams combination, key technical indexes testing research, the integrated development of composite microscopy prototype system and composite imaging are completed. The composite microscope can image the fluorescent labeled samples, which has the functions of red-green two-color fluorescence imaging, 2P green fluorescence imaging and STED super-resolution green fluorescence imaging. In terms of index, the imaging depth is 700 μm, and the resolution of STED imaging is better than 60 nm.