Spectroscopy and Spectral Analysis, Volume. 42, Issue 9, 2795(2022)

A Visual Colorimetric Method for Hydrogen Peroxide Detection Based on the Peroxidase-Like Properties of Cu(Ⅱ)

Ji MA1; 2; *;... Guo-xia HUANG1; 2;, Jun-sheng LI1; 2; *;, Liu-juan YAN1; 2; and Qian ZHANG1; |Show fewer author(s)
Author Affiliations
  • 1. Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China
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    Figures & Tables(6)
    Schematic illustration of Cu2+-H2O2-TMB colorimetric reaction (a) and colorimetric detection of H2O2 (b)
    UV-Visible absorption spectra of feasibility experiment; Inset is the typical photograph of the corresponding curvea: TMB-H2O2; b: TMB-Cu2+; c: TMB-H2O2-Cu2+; cTMB=6×10-4 mol·L-1; cCu2+=8×10-3 mol·L-1; cH2O2=1×10-5 mol·L-1
    Effect of reaction timecTMB=6×10-4 mol·L-1; cCu2+=8×10-3 mol·L-1; cH2O2=1×10-5 mol·L-1
    UV-Vis spectraofthe Cu2+-TMB- H2O2 systemcTMB=6×10-4 mol·L-1; cCu2+=8×10-3 mol·L-1; a—h: the H2O2 concentrations are 0, 0.80, 2.0, 4.0, 8.0, 16, 24, 32 and 40×10-6 mol·L-1, respectively
    The fitted curve of A652 versus the concentration of H2O2
    • Table 1. Result of H2O2 in tremella detected by this proposed method

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      Table 1. Result of H2O2 in tremella detected by this proposed method

      样品起始量/
      (μmol·L-1)
      加标量/
      (μmol·L-1)
      测定值/
      (μmol·L-1)
      回收率/
      %
      相对标准偏差
      (%, n=3)
      10.3610.0010.59102.22±1.353.02
      20.3620.0019.7797.10±0.421.26
      30.3630.0032.51107.08±1.260.52
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    Ji MA, Guo-xia HUANG, Jun-sheng LI, Liu-juan YAN, Qian ZHANG. A Visual Colorimetric Method for Hydrogen Peroxide Detection Based on the Peroxidase-Like Properties of Cu(Ⅱ)[J]. Spectroscopy and Spectral Analysis, 2022, 42(9): 2795

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    Paper Information

    Category: Research Articles

    Received: Jul. 24, 2021

    Accepted: Nov. 1, 2021

    Published Online: Nov. 17, 2022

    The Author Email:

    DOI:10.3964/j.issn.1000-0593(2022)09-2795-05

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