Infrared and Laser Engineering, Volume. 51, Issue 9, 20210915(2022)

Holographic microscopy detection method of microfluidic chip channel

Jianan Xu, Ming Kong, Wei Liu*, Daodang Wang, and Zhongsi Xie
Author Affiliations
  • College of Metrology and Measurement Engineering, China Jiliang University, Hangzhou 310018, China
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    Figures & Tables(9)
    Schematic diagram of system coordinates
    Schematic of the experimental system of reflective off-axis IPDHM
    Correction results of surface fitting method for different polynomials. (a) 6-term quadratic polynomial; (b) 9-term quadratic polynomial; (c) Zernike higher order polynomial
    Experimental results of two-step phase subtraction method for micro-step. (a) Backgroud area hologram; (b) Micro-step hologram; (c) Reconstructed amplitude of background area; (d) Reconstructed amplitude of micro-step; (e) Unwrapped phase of backgroud area; (f) Unwrapped phase of micro-step; (g) Height distribution of micro-step; (h) 3D diagram of micro-step
    Comparison of experimental results of two-step phase subtraction method and surface fitting method
    Experimental results of the microfluidic chip channel. (a) Background area hologram; (b) Channel hologram; (c) Reproducted amplitude of background area; (d) Reproducted amplitude of channel; (e) Unwrapped phase of background area; (f) Unwrapped phase of channel; (g) Depth of channel; (h) 3D diagram of channel
    Experimental results of fractured surface defect. (a) Reproducted amplitude of channel and defect; (b) Magnified view of defect’s reproducted amplitude; (c) Depth of defect; (d) Depth distribution of the horizontal line in (c); (e) 3D diagram of defect
    Experimental results of defective surface defect. (a) Reproducted amplitude of channel and defect; (b) Magnified view of defect’s reproducted amplitude; (c) Depth of channel and defect ; (d) Depth distribution of the horizontal line in (c); (d) 3D diagram of defect
    • Table 1. Comparison of micro-step height errors by each phase distortion correction method

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      Table 1. Comparison of micro-step height errors by each phase distortion correction method

      AlgorithmTwo-step phase subtraction6-term quadratic polynomial9-term quadratic polynomialZernike polynomial
      Measured value/nm64.347.746.443.9
      HRE1.1%26.6%28.6%32.5%
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    Jianan Xu, Ming Kong, Wei Liu, Daodang Wang, Zhongsi Xie. Holographic microscopy detection method of microfluidic chip channel[J]. Infrared and Laser Engineering, 2022, 51(9): 20210915

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    Paper Information

    Category: Photoelectric measurement

    Received: Dec. 29, 2021

    Accepted: --

    Published Online: Jan. 6, 2023

    The Author Email: Liu Wei (liuw@cjlu.edu.cn)

    DOI:10.3788/IRLA20210915

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