Laser & Optoelectronics Progress, Volume. 56, Issue 9, 091701(2019)

Biological Cell Phase Imaging System Integrated with Optical Scattering Information

Ying Ji1、*, Shuang Fu2, Zhaohe Tao1, Minjie Liang1, and Yawei Wang1
Author Affiliations
  • 1 Faculty of Science, Jiangsu University, Zhenjiang, Jiangsu 212013, China
  • 2 School of Mechanical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China
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    Figures & Tables(11)
    Schematic of biological cell phase imaging system integrated with optical scattering information
    Flow chart of system
    Schematic of model. (a) Homogeneous cells; (b) monocytes; (c) binuclear cells
    Phase distributions. (a) Homogeneous cells; (b) monocytes; (c) binuclear cells
    Modulus square processing of phase gradient. (a) Homogeneous cells; (b) monocytes; (c) binuclear cells
    Axial distribution of modulus square of phase gradient. (a) Homogeneous cells; (b) monocytes; (c) binuclear cells
    Scattered light intensity distributions of models and diagonal distributions of scattered light intensity. (a) (d) Homogeneous cells; (b) (e) monocytes; (c) (f) binuclear cells
    Phase information of human red blood cells. (a) Phase distribution; (b) axial distribution of modulus square of phase gradient
    Analysis diagram of human neutrophils. (a) Phase distribution; (b) axial distribution of modulus square of phase gradient
    • Table 1. Horizontal coordinates of break points in axial distribution curve

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      Table 1. Horizontal coordinates of break points in axial distribution curve

      Cell typeHorizontal coordinate of break point
      HomogeneousMonocyteA(53) B(204)A(53) B(100) C(157) D(204)
      BinuclearA(53) B(100) C(112) D(145)E(157) F(204)
    • Table 2. Comparison of measured values with model parameters

      View table

      Table 2. Comparison of measured values with model parameters

      Cell typeDesign parameter /μm(cell diameter/nuclear diameter)Measured value /μm(cell diameter/nuclear diameter)Average error rate /%
      Homogeneous18.0017.761.33
      Monocyte18.00/7.0017.76/6.941.08
      Binuclear18.00/7.00/4.0017.77/6.71/3.882.81
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    Ying Ji, Shuang Fu, Zhaohe Tao, Minjie Liang, Yawei Wang. Biological Cell Phase Imaging System Integrated with Optical Scattering Information[J]. Laser & Optoelectronics Progress, 2019, 56(9): 091701

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    Paper Information

    Category: Medical Optics and Biotechnology

    Received: Sep. 6, 2018

    Accepted: Nov. 26, 2018

    Published Online: Jul. 5, 2019

    The Author Email: Ji Ying (jy@ujs.edu.cn)

    DOI:10.3788/LOP56.091701

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