Response of Photosynthetic Fluorescence Parameters of Chlorella Pyrenoidosa to Cu <sup>2+</sup> Toxicity

Li Hu; Gaofang Yin; Nanjing Zhao; Min Chen; Tingting Gan

doi:10.3788/AOS202040.1217001

Acta Optica Sinica, Volume. 40, Issue 12, 1217001(2020)

Response of Photosynthetic Fluorescence Parameters of Chlorella Pyrenoidosa to Cu ²⁺ Toxicity

Li Hu^1,2, Gaofang Yin^2、*, Nanjing Zhao², Min Chen^2,3, and Tingting Gan²

Author Affiliations

¹College of Physics and Materials Engineering, Hefei Normal University, Hefei, Anhui 230061, China

²Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230061, China

³University of Science and Technology of China, Hefei, Anhui 230026, China

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Figures & Tables(6)

Fig. 1. Schematic diagram of chlorophyll fluorescence analyzer induced by variable light pulse

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Fig. 2. Variation trend of fluorescence parameters with time under different Cu²⁺ concentrations. (a) Yield; (b) rP; (c)α; (d) Fv/Fm

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Fig. 3. Variation trend of fluorescence parameters with time under different Cu²⁺ concentrations. (a) F₀; (b) Fm; (c) Fv; (d) JVP II; (e) Chl-a; (f) Ek

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Fig. 4. Inhibition ratio for different parameters versus Cu²⁺ concentration under 24 h stress. (a) Yield; (b) rP; (c) α; (d) Fv/Fm

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Table 1. Photosynthetic fluorescence parameters acquired by the chlorophyll fluorescence analyzer

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Table 1. Photosynthetic fluorescence parameters acquired by the chlorophyll fluorescence analyzer

Serial number	Parameter	Source of parameter	Name and significance
1	F₀	Fluorescence kinetic curve	The initial fluorescence
2	Fm	Fluorescence kinetic curve	The biggest fluorescence
3	σ_PSII	Fluorescence kinetic curve	Functional absorptioncross section of PSII
4	Q_A	Fluorescence kinetic curve	Average reduction time constantof plastoquinone
5	Fv	Fm-F₀	The variable fluorescence, which reflectsthe redox state of Q_A and other dissipatedenergy pathways
6	Fv/Fm	(Fm-F₀)/Fm	Maximum photochemicalquantum yield,which is the biggest lightenergy conversion efficiency of PSⅡ
7	Yield	(Fm'-F'₀)/Fm'	Actual quantum yieldof PSⅡunder light
8	rP	Yield×0.84×0.5	Relative photosynthetic electrontransfer efficiency of PSⅡ
9	JVPⅡ	K_R×Fm×F₀×Fq'/(Fm'×Fv'×E_LED)	PSⅡ flux per unit volume,which is associated withprimary productivity
10	NPQ	(Fm-Fm')/Fm'	Non photochemical quenching,which reflects the ability of plants to dissipateexcess light energy into heat
11	α	Fast light curve	The maximum light utilization coefficient,which reflects the light energyutilization efficiency
12	Ek	Fast light curve	The saturation light intensity,which reflects the strong lighttolerance of the sample

Table 2. Measured values and significant difference of photosynthetic fluorescence parameters under 24 h Cu²⁺ stress

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Table 2. Measured values and significant difference of photosynthetic fluorescence parameters under 24 h Cu²⁺ stress

Cu²⁺concentration /(μmol·L^-1)	0	0.5	1	2.5	5	25	50	100	250
F0	143.1	136.8	125.9^*	111.8^**	101.8^**	79.35^**	68.12^**	26.13^**	5.033^**
Fm	310.2	286.1^**	271.3^**	236.1^**	206.1^**	140.3^**	100.7^**	32.68^**	5.116^**
Fv	167.1	149.3^**	145.4^**	124.3^**	124.3^**	60.95^**	32.58^**	6.55^**	0.083^**
Fv/Fm	0.539	0.522	0.536	0.527	0.501^*	0.435^**	0.324^**	0.2^**	0.016^**
Yield	0.497	0.476	0.489	0.481	0.451	0.4^*	0.281^**	0.157^**	0.01^**
rP	18.16	18.28	18.13	17.89^*	16.76^*	14.4^**	10.04^**	5.576^**	0.854^**
JVPⅡ	4.707	4.446	4.101	3.647^*	3.307^*	2.606^**	2.111^**	0.731^**	0.11^**
Ek	417.2	401.1^**	391.8^**	405.1^**	411.4^*	372.5^**	330.1^**	217.2^**	155.4^**
α	0.541	0.545	0.542	0.534	0.531^*	0.431^**	0.302^**	0.173^**	0.027^**
Note: "" and "*" indicate differences at the level of 0.05 and 0.01, respectively. The experimental data of σ_PSII,Q_A,NPQ have no significant difference in the concentration area, so it is not listed in the table.

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Li Hu, Gaofang Yin, Nanjing Zhao, Min Chen, Tingting Gan. Response of Photosynthetic Fluorescence Parameters of Chlorella Pyrenoidosa to Cu ²⁺ Toxicity[J]. Acta Optica Sinica, 2020, 40(12): 1217001

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Paper Information

Category: Medical Optics and Biotechnology

Received: Feb. 18, 2020

Accepted: Mar. 23, 2020

Published Online: Jun. 3, 2020

The Author Email: Yin Gaofang (gfyin@aiofm.ac.cn)

DOI:10.3788/AOS202040.1217001

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Photosynthetic fluorescence parameters acquired by the chlorophyll fluorescence analyzer

Table 1. Photosynthetic fluorescence parameters acquired by the chlorophyll fluorescence analyzer

Table 2. Measured values and significant difference of photosynthetic fluorescence parameters under 24 h Cu2+ stress

Table 2. Measured values and significant difference of photosynthetic fluorescence parameters under 24 h Cu2+ stress

Table 2. Measured values and significant difference of photosynthetic fluorescence parameters under 24 h Cu²⁺ stress

Table 2. Measured values and significant difference of photosynthetic fluorescence parameters under 24 h Cu²⁺ stress