Research on Soybean Bacterial Disease Markers Based on Raman Spectroscopy

Yu HAN; Shao-zhong SONG; Jia-huan ZHANG; Yong TAN; Chun-yu LIU; Yun-quan ZHOU; Guan-nan QU; Yan-li HAN; Jing ZHANG; Yu HU; Wei-shi MENG; Huan-jun LIU; Yi-xiang ZHANG; Jia-yi LI

doi:10.3964/j.issn.1000-0593(2022)02-0459-05

Spectroscopy and Spectral Analysis, Volume. 42, Issue 2, 459(2022)

Research on Soybean Bacterial Disease Markers Based on Raman Spectroscopy

Yu HAN^1、1;, Shao-zhong SONG^{2、2; *;}, Jia-huan ZHANG^3、3;, Yong TAN^{1、1; *;}, Chun-yu LIU^1、1;, Yun-quan ZHOU^1、1;, Guan-nan QU^1、1;, Yan-li HAN^4、4;, Jing ZHANG^3、3;, Yu HU^3、3;, Wei-shi MENG^3、3;, Huan-jun LIU^5、5;, Yi-xiang ZHANG^1、1;, and Jia-yi LI^1、1;

Author Affiliations

¹1. Jilin Province Key Laboratory of Spectral Detection Science and Technology, School of Science, Changchun University of Science and Technology, Changchun 130022, China

²2. School of Information Engineering, Jilin Normal University of Engineering and Technology, Changchun 130052, China

³3. College of Plant Protection, Jilin Agricultural University, Changchun 130118, China

⁴4. School of Aviation Operations Service, Naval Aviation University, Yantai 264000, China

⁵5. Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Changchun 130012, China

show less

Abstract Get PDF(in Chinese)

Figures & Tables(5)

Fig. 1. The optimized molecular configuration and theoretical Raman spectrum of N-acetylmuramic acid

Download full size

View in Article

Fig. 2. The (a) theory of N-acetylmuramic acid in the range of 200~1 650 cm^-1, (b) experimentally measured N-acetylmuramic acid standard CRS and (c) the pure cultured soybean bacterial pathogen strain PSG Raman spectrum comparison

Download full size

View in Article

Fig. 3. Fitting of partial superimposed peaks of Raman spectra of pathogens of soybean bacterial diseases

Download full size

View in Article

Table 1. Optimized geometrical parameters of N-acetylmuramic acid

View table

View in Article

Table 1. Optimized geometrical parameters of N-acetylmuramic acid

Tag	Symbol	Bond/Å	Angle/(°)	Dihedral/(°)
1	C
2	C	2.35
3	C	1.33	91.412 2
4	C	1.49	121.366 9	12.769 6
5	C	1.35	93.167 7	-0.916 6
6	O	1.36	124.143 0	-8.347 5
7	O	1.34	124.111 9	174.588 2
8	H	0.96	108.139 3	177.748 1
9	O	3.54	83.794 0	-111.844 0
10	H	0.96	150.919 7	56.716 5
11	O	1.48	110.946 8	95.682 9
12	N	1.41	121.935 0	1.750 8
13	H	1.01	117.650 7	115.873 0
14	C	1.38	129.199 7	-68.546 5
15	O	1.22	119.986 6	-178.902 0
16	C	1.43	116.049 6	66.898 0
17	H	1.09	109.563 3	44.009 8
18	C	1.54	114.844 8	-73.684 5
19	O	1.34	118.533 8	-41.087 6
20	H	0.99	114.120 5	0.894 4
21	O	1.20	120.260 2	140.925 7
22	C	1.51	117.013 1	1.485 1
23	H	1.09	109.991 2	73.988 1
24	H	1.08	107.889 0	-167.027 0
25	H	1.09	111.999 7	-45.789 7
26	C	1.52	107.443 5	163.380 5
27	H	1.09	110.186 3	-178.528 0
28	H	1.09	109.957 8	-58.311 8
29	H	1.09	110.555 2	61.662 4
30	H	3.11	86.362 6	-91.880 9
31	H	3.85	86.961 9	-91.797 0
32	H	1.09	112.259 1	-146.766 0
33	H	1.08	117.638 3	-174.625 0
34	C	1.50	142.638 5	-9.875 5
35	H	1.09	110.646 7	75.595 2
36	H	1.10	107.350 1	-40.547 7
37	O	1.40	112.161 2	-165.243 0
38	H	0.97	108.346 7	58.798 6
39	H	0.97	70.634 4	-32.842 1

Table 2. Experimental and theoretical vibration frequency (cm^-1) and attribution of N-acetylmuramic acid

View table

View in Article

Table 2. Experimental and theoretical vibration frequency (cm^-1) and attribution of N-acetylmuramic acid

Theoretical/cm^-1	Standard/cm^-1	Experimental/cm^-1 (FWHM)	Assignments
222.0	221.0	229.0(30.0)	ρ(1C—H 2C—H)
284.0	289.0	287.5(49.5)	ρ(7O—H 90—H)
355.0	354.0	354.0(30.0)	ρ(26C—H 18C=21O) δ(5C—12N—14C)
621.0	621.5	621.0(11.5)	ρ(14C=15O 12N—H)
674.0	665.0	668.0(24.5)	ρ(4C—H ) ν_s(5C—1C)
718.5	723.0	724.5(4.0)	ν_s(2C—34C 34C—37O)
851.5	859.0	847.0(81.5)	ρ(3C—H 26C—H)
905.0	907.0	912.0(98.5)	ρ(26C—H 11O—4C)
931.5	930.0	927.0(51.0)	ν_s(4C—11O—16C)
1 011.5	1 009.0	1 005.5(10.0)	τ(22C—H) ν(14C—12N)
1 038.0	1 038.0	1 034.0(12.0)	ρ(7O—H 3C—H)
1 091.0	1 099.0	1 104.5(12.0)	ν_s(34C—37O ) ρ(5C—H)
1 419.5	1 422.5	1 419.0(35.5)	ρ(34C—H 37O—H)
1 481.5	1 469.0	1 461.0(44.0)	ρ(26C—H )
1 623.5	1 619.0	1 612.5(12.0)	ρ(4C—H 9O—H)

Tools

Get Citation

Copy Citation Text

Yu HAN, Shao-zhong SONG, Jia-huan ZHANG, Yong TAN, Chun-yu LIU, Yun-quan ZHOU, Guan-nan QU, Yan-li HAN, Jing ZHANG, Yu HU, Wei-shi MENG, Huan-jun LIU, Yi-xiang ZHANG, Jia-yi LI. Research on Soybean Bacterial Disease Markers Based on Raman Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2022, 42(2): 459

Download Citation

EndNote(RIS)BibTex Plain Text

Set citation alerts for article

Save article for my favorites

Paper Information

Category: Research Articles

Received: Jan. 20, 2021

Accepted: Feb. 17, 2021

Published Online: Apr. 2, 2022

The Author Email:

DOI:10.3964/j.issn.1000-0593(2022)02-0459-05

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Optimized geometrical parameters of N-acetylmuramic acid

Table 1. Optimized geometrical parameters of N-acetylmuramic acid

Table 2. Experimental and theoretical vibration frequency (cm-1) and attribution of N-acetylmuramic acid

Table 2. Experimental and theoretical vibration frequency (cm-1) and attribution of N-acetylmuramic acid

Table 2. Experimental and theoretical vibration frequency (cm^-1) and attribution of N-acetylmuramic acid

Table 2. Experimental and theoretical vibration frequency (cm^-1) and attribution of N-acetylmuramic acid