Laser & Optoelectronics Progress, Volume. 61, Issue 6, 0618010(2024)
Research Progress of Three-Dimensional Raman Microscopic Imaging (Invited)
Raman microscopic imaging has emerged as an important research tool in life sciences because it does not require sample preparation and is non-destructive, non-invasive, and insensitive to aqueous solutions. It enables the characterization of biochemical components and sample distributions at a micrometer or nanometer scale. As the research on complex biological samples increases, Raman microscopic imaging holds potential for the dynamic stereoscopic observation of molecular composition and distribution in biological samples. This paper systematically examined recent advancements in 3D Raman microscopic imaging, including technical approaches, improvement strategies, and experimental results of various 3D imaging methods based on spontaneous Raman scattering, coherent Raman scattering, surface-enhanced Raman scattering, and Raman tags. Further, the progress of the applications of different imaging techniques in cell biology and developmental biology was summarized. Finally, the challenges and development prospects of different 3D Raman microscopic imaging techniques in the application of biomedical optical microscopic imaging technology were outlined.
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Gong Feng, Tingyan Xing, Nan Wang, Qi Zeng, Hui Xie, Xueli Chen. Research Progress of Three-Dimensional Raman Microscopic Imaging (Invited)[J]. Laser & Optoelectronics Progress, 2024, 61(6): 0618010
Category: Microscopy
Received: Dec. 7, 2023
Accepted: Jan. 8, 2024
Published Online: Mar. 29, 2024
The Author Email: Wang Nan (xlchen@xidian.edu.cn), Chen Xueli (wangnan@xidian.edu.cn)