Journal of Inorganic Materials, Volume. 35, Issue 1, 131(2020)

Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2

Bao-Kai MA1...2,3, Mian LI3, Ling-Zhi CHEONG2,*, Xin-Chu WENG1, Cai SHEN3 and Qing HUANG3 |Show fewer author(s)
Author Affiliations
  • 1School of Life and Sciences, Shanghai University, Shanghai 200444, China
  • 2Department of Food Science and Engineering, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China
  • 3Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo 315201, China
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    Figures & Tables(11)
    Schematic illustration for fabrication of HRP@MXene (Graphite/TiC/Ti3C2)/chitosan/GCE and H2O2 sensing principle of HRP@MXene/chitosan/GCE
    XRD patterns of G/TiC/Ti3AlC2 and G/TiC/Ti3C2 (A); FT-IR spectra of the MXene, HRP and HRP@MXene (B); SEM images of the MXene G/TiC (C) and Ti3C2 (D)
    EIS of Chit(chitosan)/GCE(a), MXene/Chit/GCE(b), HRP@MXene/Chit/GCE (c) electrodes cycled in 0.1 mol•L-1 KCL aqueous solution containing 5 mmol•L-1 [Fe(CN)6]3-/4- (A); CV curves of Chit/GCE (a), MXene/Chit/GCE (b), HRP@MXene/Chit/GCE (c) electrodes cycled in 0.1 mol•L-1 KCL aqueous solution containing 5 mmol•L-1 [Fe(CN)6]3-/4-: (potential window: -0.1-0.5 V vs. SCE) (B)
    CV curves of Chit/GCE (curve a, black line), MXene/ Chit/GCE (curve b, red line), HRP/Chit/GCE (curve c, pink line), HRP@MXene/Chit/GCE (curve d, blue line) electrodes cycled in N2-saturated 0.1 mol•L-1 PBS (pH 7.5) containing 1.0 mmol•L-1 HQ and 2.0 mmol•L-1 H2O2 at a scanning rate of 50 mV•s-1 (potential window: -0.8-0.8 V vs. SCE).
    Amperometric responses of HRP@MXene/Chit/ GCE at -0.1 V upon successive additions of H2O2 in astirred 0.1 mol•L-1 PBS (pH 7.5) (A); Calibration curve of amperometric responses at different H2O2 concentrations (B); Amperometric responses of HRP@MXene/Chit/ GCE at -0.1 V upon successive additions of solutions extracted from milk sample (C) and dried scallop (D) spiked with different H2O2 under stirred 0.1 mol•L-1 PBS (pH 7.5)
    EIS of various electrodes in 0.1 mol•L-1 KCL aqueous solution containing 5 mmol•L-1 [Fe(CN)6]3-/4-: Chit (pH 5.0)/GCE (curve b, red line), Chit (pH 6.0)/GCE (curve c, blue line) , Chit (pH 6.5)/GCE (curve d, green line), Chit (pH 7.0)/GCE (curve e, pink line) (A); CV curves of Chit (pH 5.0)/GCE (curve b, red line), Chit (pH 6.0)/GCE (curve c, blue line) , Chit (pH 6.5)/GCE (curve d, green line) , Chit (pH 7.0)/GCE (curve e, pink line) electrodes cycled in 0.1 mol•L-1 KCL aqueous solution containing 5 mmol•L-1 [Fe(CN)6]3-/4-: (potential window: -0.1-0.5 V vs. SCE) (B)
    CV curves of HRP@MXene/Chit/GCE electrodes cycled in N2-saturated 0.1 mol•L-1 PBS (pH 7.5) containing 1.0 mmol•L-1 HQ and 2.0 mmol•L-1 H2O2 at a different scanning rates (20-500 mV•s-1) (A); Plot of cathodic and anodic peak current for HRP@MXene/Chit/GCE versus scanning rate (B); Inset: Plots of anodic peak potential and cathodic peak potential for HRP@MXene/Chit/GCE electrode versus the logarithm of scan rate
    Effects of PBS buffer’s pH (A) and concentration of MXene (B) on the cathodic peak current of enzyme biosensor cycled in N2-saturated 0.1 mol•L-1 PBS ( pH 7.5) containing 1.0 mmol•L-1 HQ and 2.0 mmol•L-1 H2O2; Effects of PBS buffer’s pH (C) and concentration of MXene (D) on the DPV response of enzyme biosensor cycledin N2-saturated 0.1 mol•L-1 PBS (pH 7.5) containing 1.0 mmol•L-1 HQ and 2.0 mmol•L-1 H2O2
    Amperometric response of HRP@MXene/Chit/GCE in 0.1 mol•L-1 pH 7.5 PBS containing 100 mmol•L-1 of ascorbic acid, glucose, uric acid and H2O2 (Applied potential: -0.1 V) (A); Reduction peak currents of HRP@MXene/Chit/GCE stored in 50 mmol•L-1 PBS (pH 7.5) at 4 for 10 d (B)
    • Table 1.

      Comparison of the performance of present work with other published electrodes for hydrogen peroxide detection

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      Table 1.

      Comparison of the performance of present work with other published electrodes for hydrogen peroxide detection

      ElectrodeLinear range/(mmol•L-1)LOD/(mmol•L-1)Ref.
      HRP-CTAB-Au/GCE0.50-1050.23[1]
      HRP/GO/GCE0.002-0.51.6[2]
      HRP/TB/CCB0.429-4550.17[3]
      HRP-BMIM·BF4/SWCNTs0.49 to 10.20.13[4]
      HRP/PGN/GCE2.77-8352.67 ×10-4[5]
      Hb-MXene-GO/Au foil2-1×1031.95[6]
      MXene/GCE-0.7×10-3[7]
      Hb-naf-MXene/GCE0.1-2600.02[8]
      TiO2-Hb-naf-MXene/GCE0.1-3801.4×10-2[9]
      HRP@MXene/Chitosan/GCE5-1.65×1030.74This work
    • Table 2.

      Detection of hydrogen peroxide in real food sample

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      Table 2.

      Detection of hydrogen peroxide in real food sample

      SampleAdded H2O2/(mmol•L-1)Found H2O2/(mmol•L-1)Recovery/%RSD/%
      Milk12.513.037104.305.88
      Milk5052.57105.141.12
      Milk125136.5109.203.33
      Dried scallop066.56--
      Dried scallop12.577.8490.246.97
      Dried scallop50120.08107.041.46
      Dried scallop125189.1198.048.39
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    Bao-Kai MA, Mian LI, Ling-Zhi CHEONG, Xin-Chu WENG, Cai SHEN, Qing HUANG. Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2[J]. Journal of Inorganic Materials, 2020, 35(1): 131

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    Paper Information

    Category: RESEARCH LETTERS

    Received: Mar. 28, 2019

    Accepted: --

    Published Online: Feb. 24, 2021

    The Author Email: CHEONG Ling-Zhi (lingzhicheong@yahoo.com)

    DOI:10.15541/jim20190139

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