Journal of Radiation Research and Radiation Processing, Volume. 41, Issue 2, 020302(2023)
Protective effects of a Chinese medicinal formula on the spleen of irradiated mice
Figures & Tables(9)
Fig. 1. Pathological pictures of HE staining in different groups of mice spleen tissue (color online)
Fig. 2. Expression of Bax, Bcl-2 and P53 proteins in the spleen of mice in each group: (a) western blot showing expression of Bax,Bcl-2 and P53 proteins in the spleen; (b) quantitative analysis of Bax, Bcl-2 and P53;
Fig. 4. Anti-apoptotic mechanism of Fangfu formulas decoction on spleen of mice with radiation injury
Table 1.
Changes of weight and spleen index of mice in each group View tableView in ArticleTable 1.
Changes of weight and spleen index of mice in each group 组别
Groups
初始体重 / g
Initial weight
末次体重 / g
Last weight
脾脏系数 / (mg·g-1)
Spleen index
对照组Control group 24.37±1.56 36.97±1.11** 0.031±0.009** 模型组Model group 24.93±1.03 31.28±3.33## 0.009±0.002## 低剂量组Low-dose group 23.79±1.31 32.45±2.96## 0.010±0.002## 中剂量组Middle-dose group 24.56±1.78 36.15±1.32** 0.024±0.007** 高剂量组High-dose group 23.77±0.63 34.95±2.43##* 0.014±0.004##**
Table 2.
Pathological score of the spleen tissue of the mice in each group View tableView in ArticleTable 2.
Pathological score of the spleen tissue of the mice in each group 组别Groups 坏死Necrosis 中性粒细胞浸润Neutrophil infiltration 对照组Control group 0.30±0.48 0.30±0.48 模型组Model group 2.30±0.95## 3.51±0.71## 低剂量组Low-dose group 1.71±0.167 1.40±0.52** 中剂量组Middle-dose group 0.92±0.88** 1.32±0.82** 高剂量组High-dose group 0.41±0.52** 0.61±0.52**
Table 3.
Effect of Fangfu Formulass on Caspase-3, Caspase-9 and cytochrome C activity in spleen cells View tableView in ArticleTable 3.
Effect of Fangfu Formulass on Caspase-3, Caspase-9 and cytochrome C activity in spleen cells 组别Groups Caspase-3 / (ng·mL-1) Caspase-9 / (ng·mL-1) Cytochrome C / (nmol·mL-1) 对照组Control group 2.98±0.03** 1.49±0.01** 22.87±0.83** 模型组Model group 3.54±0.10## 1.62±0.03## 34.32±2.42## 低剂量组Low-dose group 3.10±0.11##** 1.57±0.02##** 26.55±1.37##** 中剂量组Middle-dose group 3.05±0.02** 1.53±0.01** 23.12±0.77** 高剂量组High-dose group 2.98±0.02** 1.50±0.08* 20.90±0.18**
Table 4.
Effect of Fangfu formulas on T lymphocyte subsets in spleen of mice in each group View tableView in ArticleTable 4.
Effect of Fangfu formulas on T lymphocyte subsets in spleen of mice in each group 组别Groups CD4+ / % CD8+ / % CD4+ / CD8+ 对照组Control group 53.10±15** 16.80±3.23** 3.16±1.47** 模型组Model group 14.88±5.83## 8.38±5.45## 1.78±0.46## 低剂量组Low-dose group 42.45±3.32#** 16.33±0.58#** 2.60±0.03#* 中剂量组Middle-dose group 44.95±19.59** 16.64±0.78** 2.71±1.34** 高剂量组High-dose group 45.85±1.06** 16.73±0.88** 2.74±0.86**
Table 5.
Effects of Fufang capsules on DNA damage in spleen cells of mice in each group View tableView in ArticleTable 5.
Effects of Fufang capsules on DNA damage in spleen cells of mice in each group 组别Groups Tail DNA / % Tail length / μm OTM / μm 对照组Control group 8.14±0.52** 9.10±1.07** 1.07±0.83** 模型组Model group 20.02±2.11## 20.60±3.22## 3.76±1.65## 低剂量组Low-dose group 14.34±2.51##** 14.80±1.89##** 2.29±1.36##** 中剂量组Middle-dose group 13.81±1.87** 13.60±2.10** 2.10±0.82** 高剂量组High-dose group 18.76±1.66** 19.38±1.52* 3.85±0.91**
Tools
Get Citation
Copy Citation Text
Xi ZHOU, Ying TANG, Huan SUN, Xuejiao CHEN. Protective effects of a Chinese medicinal formula on the spleen of irradiated mice[J]. Journal of Radiation Research and Radiation Processing, 2023, 41(2): 020302
Paper Information
Category: Research Articles
Received: Aug. 26, 2022
Accepted: Jan. 19, 2023
Published Online: Jul. 24, 2023
The Author Email: TANG Ying (1446329418@qq.com)
© Copyright 2018-2021 | Chinese Laser Press.
All Rights Reserved 沪ICP备15018463号-20