Laser & Optoelectronics Progress, Volume. 59, Issue 23, 2317001(2022)

Effect of Nano-Film Substrates on Ciprofloxacin Detection by Surface-Enhanced Raman Spectroscopy

Yiqian Guo1, Hongyan Wang2, Miao Qin2, Yida Zeng1、*, Li Xia3, and Yangpo Hu3
Author Affiliations
  • 1School of Aeronautical Manufacturing Engineering, Nanchang Hangkong University, Nanchang 330063, Jiangxi, China
  • 2School of Chemistry & Chemical Engineering, Suzhou University, Suzhou 234099, Anhui, China
  • 3Jiangxi Hongdu Aviation Industry Co., Ltd., Nanchang 330024, Jiangxi, China
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    Figures & Tables(19)
    Schematic diagram of assembly process of gold nano-colloid enhanced substrate. (a) 5 µL of gold nano-colloids are dropped onto silicon wafer as substrate; (b) volume of gold colloids decrease during natural drying; (c) gold colloids are dried completely to form golden films on silicon wafers
    SERS pretreatment. (a) Original SERS; (b) LabSpec software operation interface; (c) SERS after pretreatment
    UV-vis spectra of self-made gold colloids. (a) Au NPs; (b) Au NRs
    SEM morphology of gold nanoparticles. (a) Au NPs and particle size distribution; (b) Au NRs
    Normal Raman spectra (NRS) of CIP
    SERS of CIP based on different laser wavelengths
    NRS, SERS based on Au NPs substrate of CIP aqueous solution and Raman spectra of Au NPs substrate
    SERS based on Au NRs substrate of CIP aqueous solution and Raman spectra of Au NRs substrate substrate
    Stability research atlas. (a) Au NPs substrate; (b) Au NRs substrate
    SERS of chicken extracts containing 2.0×10-6 CIP detected based on different enhanced substrates
    SERS images of different concentrations of CIP solution detected based on Au NPs substrate
    Working curves of CIP solutions with different concentrations detected based on Au NPs substrate. (a) 1167 cm-1; (b) 1378 cm-1
    SERS images of different concentrations of CIP solution detected based on Au NRs substrate
    Working curves of CIP solutions with different concentrations detected based on Au NRs substrate. (a) 1142 cm-1;(b) 1269 cm-1; (c) 1389 cm-1
    • Table 1. Main peak attribution of NRS, SERS based on Au NPs substrate and Au NRs substrate of CIP

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      Table 1. Main peak attribution of NRS, SERS based on Au NPs substrate and Au NRs substrate of CIP

      NRS /cm-1SERS based on Au NPs /cm-1SERS based on Au NRs /cm-1Attribution of characteristic peaks
      709709707γ(C―C)ring + γ(C=O)ring
      790786782γ(C―N)ring + γ(C―H)ring
      102610321039δ(C―N)ring + δ(C―C)ring + δ(CH2rock
      1136-1142v(C=O)ring
      11721167-δ(CH2twist
      126712611269ring breathing
      138413781389vas(C―C)benzene + v(C―H)rock
      16201630-v(C=O)ring + v(C=C)ring
    • Table 2. Standard curves of CIP standard solution under different Raman displacements measured based on Au NPs substrate

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      Table 2. Standard curves of CIP standard solution under different Raman displacements measured based on Au NPs substrate

      Raman shift /cm-10.1×10-60.5×10-61×10-65×10-610×10-6Standard curveCoefficient of determination(R2Root mean squared error(RMSE)
      116747383994715902753y = 210.48x + 621.610.9806111.6517
      13786531028111928114289y = 362.28x + 777.240.9902136.2291
    • Table 3. Recovery parameters of CIP solution detected based on Au NPs substrate

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      Table 3. Recovery parameters of CIP solution detected based on Au NPs substrate

      Raman shift /cm-1Working curveActual content /10-6SERS intensity /arb.unitsPredicted content /10-6Recovery rate /%
      1167y = 210.48x + 621.610.26450.1155.0
      0.77620.6795.7
      2.010772.16108.4
      1378y = 362.28x + 777.240.28510.21105.0
      0.710230.6897.1
      2.015182.04102.3
    • Table 4. Standard curves of CIP standard solution under different Raman displacements measured based on Au NRs substrate

      View table

      Table 4. Standard curves of CIP standard solution under different Raman displacements measured based on Au NRs substrate

      Raman shift /cm-10.1×10-60.5×10-61×10-65×10-610×10-6Standard curveR2RMSE
      114260781091815072361y = 167.75x + 683.690.992555.15
      1269832848104418042584y = 179.9x + 825.140.993953.01
      13895856617129631175y = 57.166x + 629.410.974335.01
    • Table 5. Recovery parameters of CIP solution detected based on Au NRs substrate

      View table

      Table 5. Recovery parameters of CIP solution detected based on Au NRs substrate

      Raman shift /cm-1Working curveActual content /10-6SERS intensity /arb. unitsPredicted content /10-6Recovery rate /%
      1142y = 167.75x + 683.690.27220.22111.5
      0.78080.74106.0
      2.011292.65132.5
      1269y = 179.9x + 825.140.28590.1996.3
      0.79600.75107.8
      2.012632.44121.8
      1389y = 57.166x + 629.410.26480.32161.4
      0.76871.00143.3
      2.07792.61130.4
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    Yiqian Guo, Hongyan Wang, Miao Qin, Yida Zeng, Li Xia, Yangpo Hu. Effect of Nano-Film Substrates on Ciprofloxacin Detection by Surface-Enhanced Raman Spectroscopy[J]. Laser & Optoelectronics Progress, 2022, 59(23): 2317001

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    Paper Information

    Category: Medical Optics and Biotechnology

    Received: Oct. 19, 2021

    Accepted: Nov. 29, 2021

    Published Online: Nov. 28, 2022

    The Author Email: Zeng Yida (394764103@qq.com)

    DOI:10.3788/LOP202259.2317001

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