Research Progress of Spectroscopy Detection Technologies for Waterborne Pathogens

Yu-xia HU; Jie CHEN; Hui SHAO; Pu YAN; Heng XU; Long SUN; Xiao XIAO; Lei XIU; Chun FENG; Ting-ting GAN; Nan-jing ZHAO

doi:10.3964/j.issn.1000-0593(2022)09-2672-07

Spectroscopy and Spectral Analysis, Volume. 42, Issue 9, 2672(2022)

Research Progress of Spectroscopy Detection Technologies for Waterborne Pathogens

Yu-xia HU^1、*, Jie CHEN^1、1;, Hui SHAO^1、1;, Pu YAN^1、1;, Heng XU^1、1;, Long SUN^1、1;, Xiao XIAO^1、1;, Lei XIU^3、3;, Chun FENG^2、2;, Ting-ting GAN^2、2;, and Nan-jing ZHAO^{2、2; *;}

Author Affiliations

¹1. School of Electronic and Information Engineering, Anhui Jianzhu University, Hefei 230601, China

²2. Key Laboratory of Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China

³3. School of Advanced Manufacturing Engineering, Hefei University, Hefei 230601, China

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Figures & Tables(7)

Fig. 1. Spectral ranges for pathogen monitoring

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Fig. 2. Flow diagram of spectra analysis

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Fig. 3. Different methods for measuring optical density using light

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Fig. 4. Basic principle of fluorescence

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Fig. 5. Different modes of IR spectroscopy

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Table 1. Current water pathogen detection technology and its advantages and disadvantages

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Table 1. Current water pathogen detection technology and its advantages and disadvantages

技术	方法	优点	缺点	参考文献
培养技术	滤膜法多管发酵法平板计数法	准确性高	检测时间长, 制备过程繁琐, 需要培养病原菌	[8-12]
分子生物学技术	聚合酶链反应法 (PCR)	分析速度较快、灵敏度高、特异性强、测试简单	难以区分微生物的活性、需专人操作、设备要求高	[13-14]
分子生物学技术	原位荧光杂交法(FISH)	监测速度较快、快速识别不同病原菌、能区分微生物活性	需要昂贵的荧光探针、易受病原菌自身荧光干扰	[13-14]
免疫学技术	酶联免疫吸附法	监测速度较快、操作简便、特异性强、灵敏度高	检测范围复杂且窄、存在交叉反应	[15-17]
	免疫磁分离法(IMS)	高效、特异性好	成本较高
	酶联荧光免疫分析法	所需试剂少、灵敏性更高	成本高, 检测技术复杂

Table 2. Excitation wavelength and emission wavelength of main fluorescent substances in the pathogen
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Table 2. Excitation wavelength and emission wavelength of main fluorescent substances in the pathogen
荧光物质激发波长/nm 发射波长/nm
色氨酸 240~280 280~350
酪氨酸 240~280 280~350
苯丙氨酸 240~280 280~350
NADH 340 450
核黄素 450 515~565

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Yu-xia HU, Jie CHEN, Hui SHAO, Pu YAN, Heng XU, Long SUN, Xiao XIAO, Lei XIU, Chun FENG, Ting-ting GAN, Nan-jing ZHAO. Research Progress of Spectroscopy Detection Technologies for Waterborne Pathogens[J]. Spectroscopy and Spectral Analysis, 2022, 42(9): 2672

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Paper Information

Category: Research Articles

Received: Aug. 1, 2021

Accepted: Nov. 14, 2021

Published Online: Nov. 17, 2022

The Author Email: HU Yu-xia (yxhuhf@163.com)

DOI:10.3964/j.issn.1000-0593(2022)09-2672-07

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Current water pathogen detection technology and its advantages and disadvantages

Table 1. Current water pathogen detection technology and its advantages and disadvantages

Table 2. Excitation wavelength and emission wavelength of main fluorescent substances in the pathogen

Table 2. Excitation wavelength and emission wavelength of main fluorescent substances in the pathogen