Acta Optica Sinica, Volume. 41, Issue 20, 2018001(2021)
Light Sheet Fluorescence Microscope Based on Line-Scanning Imaging
Fig. 1. Schematic of LSFM based on LSI
Fig. 2. Schematic diagram of system imaging process
Fig. 3. Control principle of LSFM based on LSI
Fig. 4. Sequence chart of synchronization system control signal
Fig. 5. Stability test of LSFM based on LSI. (a) Schematic of a single frame Gaussian beam; (b) statistic diagram of peak position of Gaussian beam in LSI process
Fig. 6. Comparison of DSLM and LSFM based on LSI images in the high scattering situation. (a) Image acquired by DSLM; (b) image acquired by LSFM based on LSI; (c)(d) higher magnification of the region indicated in Fig. (a) and (b); (e) normalized intensity values along the lines in Fig. (c) and (d)
Fig. 7. Comparison of DSLM and LSFM based on LSI in the high scattering situation. (a) Image acquired by DSLM; (b) image acquired by LSFM based on LSI; (c)(d) higher magnification of the region indicated in Fig. (a) and (b); (e) normalized intensity values along the lines in Fig. (c) and (d)
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Yueqian Wu, Xin Xu, Jixiang Wang, Xin Zhang, Guohua Shi. Light Sheet Fluorescence Microscope Based on Line-Scanning Imaging[J]. Acta Optica Sinica, 2021, 41(20): 2018001
Category: Microscopy
Received: Mar. 1, 2021
Accepted: May. 6, 2021
Published Online: Sep. 30, 2021
The Author Email: Zhang Xin (zhangx@sibet.ac.cn), Shi Guohua (ghshi_lab@126.com)