Acta Optica Sinica, Volume. 41, Issue 20, 2018001(2021)

Light Sheet Fluorescence Microscope Based on Line-Scanning Imaging

Yueqian Wu1, Xin Xu2, Jixiang Wang2, Xin Zhang2、**, and Guohua Shi2、*
Author Affiliations
  • 1Academy for Engineering and Technology, Fudan University, Shanghai 200433, China
  • 2Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, China
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    Figures & Tables(7)
    Schematic of LSFM based on LSI
    Schematic diagram of system imaging process
    Control principle of LSFM based on LSI
    Sequence chart of synchronization system control signal
    Stability test of LSFM based on LSI. (a) Schematic of a single frame Gaussian beam; (b) statistic diagram of peak position of Gaussian beam in LSI process
    Comparison of DSLM and LSFM based on LSI images in the high scattering situation. (a) Image acquired by DSLM; (b) image acquired by LSFM based on LSI; (c)(d) higher magnification of the region indicated in Fig. (a) and (b); (e) normalized intensity values along the lines in Fig. (c) and (d)
    Comparison of DSLM and LSFM based on LSI in the high scattering situation. (a) Image acquired by DSLM; (b) image acquired by LSFM based on LSI; (c)(d) higher magnification of the region indicated in Fig. (a) and (b); (e) normalized intensity values along the lines in Fig. (c) and (d)
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    Yueqian Wu, Xin Xu, Jixiang Wang, Xin Zhang, Guohua Shi. Light Sheet Fluorescence Microscope Based on Line-Scanning Imaging[J]. Acta Optica Sinica, 2021, 41(20): 2018001

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    Paper Information

    Category: Microscopy

    Received: Mar. 1, 2021

    Accepted: May. 6, 2021

    Published Online: Sep. 30, 2021

    The Author Email: Zhang Xin (zhangx@sibet.ac.cn), Shi Guohua (ghshi_lab@126.com)

    DOI:10.3788/AOS202141.2018001

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