An updated light sheet fluorescence microscope (LSFM), combining the digital scanning light sheet fluorescence microscope (DSLM) and the line-scanning imaging (LSI) method, is designed to improve the imaging quality by decreasing sample scattering. Based on DSLM, a scanning galvanometer for descanning is added in the detection light path. Moreover, the scanning galvanometer in the illumination light path is synchronized with the descanning galvanometer to fix the evenly moving images at the same position in front of the camera during the control process, thereby realizing LSI. The linear array detector is simulated by the area array camera in the system for conveniently comparing with the traditional method. In addition, compared with the conventional LSFM, the improved system is more effective in suppressing the sample scattering problem in the imaging experiments of high-scattering fluorescent microsphere samples and zebrafish heart samples. Hence, the feasibility of LSFM based on LSI method can be verified.