Opto-Electronic Engineering, Volume. 49, Issue 2, 210336-1(2022)
Rapid preparation of high-precision hydrogel micropatterns and its induction of cell behavior
Fig. 1. Schematic diagram for the preparation process of large-area hydrogel micropatterns
Fig. 2. Schematic diagram of the chemical structures of the component of photoresist. (a) Monomer; (b) Crosslinker; (c) Photoinitiators
Fig. 3. SEM images of large-size microstructures. (a)~(d) The designed pattern of large-size microstructures; (e)~(h) SEM images of large-size micropatterns; (i)~(l) Enlarged local details of large-size micropatterns
Fig. 4. Small-size microstructures prepared under different exposure conditions. (a) Laser power is 50 μW~60 μW, exposure time is 700 ms; (b) Laser power is 120 μW~140 μW, exposure time is 1500 ms
Fig. 5. (a)~(h) The bright field images of contact angle and (i) contact angle analysis of microstructures with different sizes
Fig. 6. Fluorescence and SEM images of cells cultured on large-size microstructures after 48 h. (a) Confocal fluorescence images, blue: nuclei stained by DAPI, excitation wavelength is 405 nm, collected wavelength range is 410 nm~460 nm, red: actin stained ActinRed 555, excitation wavelength is 561 nm, collected wavelength range is 570 nm~620 nm; (b) SEM images and (c) magnified SEM images of local details
Fig. 7. Fluorescence and SEM images of cells cultured on small-size microstructures after 48 h. (a)~(d) 3D confocal fluorescence images, blue: nuclei stained by DAPI, excitation wavelength is 405 nm, collected wavelength range is 410 nm~460 nm, red: actin stained ActinRed 555, excitation wavelength is 561 nm, collected wavelength range is 570 nm~620 nm; (e)~(h) SEM images
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Weicai Zhang, Meiling Zheng, Xianzi Dong, Jie Liu, Feng Jin. Rapid preparation of high-precision hydrogel micropatterns and its induction of cell behavior[J]. Opto-Electronic Engineering, 2022, 49(2): 210336-1
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Received: Oct. 21, 2021
Accepted: --
Published Online: Apr. 6, 2022
The Author Email: Zheng Meiling (zhengmeiling@mail.ipc.ac.cn)