Opto-Electronic Engineering, Volume. 49, Issue 2, 210336-1(2022)

Rapid preparation of high-precision hydrogel micropatterns and its induction of cell behavior

Weicai Zhang1...2, Meiling Zheng1,*, Xianzi Dong1, Jie Liu1 and Feng Jin1 |Show fewer author(s)
Author Affiliations
  • 1Laboratory of Organic NanoPhotonics and CAS Key Laboratory of Bio-Inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
  • 2School of Future Technology, University of Chinese Academy of Sciences, Beijing 101407, China
  • show less
    Figures & Tables(7)
    Schematic diagram for the preparation process of large-area hydrogel micropatterns
    Schematic diagram of the chemical structures of the component of photoresist. (a) Monomer; (b) Crosslinker; (c) Photoinitiators
    SEM images of large-size microstructures. (a)~(d) The designed pattern of large-size microstructures; (e)~(h) SEM images of large-size micropatterns; (i)~(l) Enlarged local details of large-size micropatterns
    Small-size microstructures prepared under different exposure conditions. (a) Laser power is 50 μW~60 μW, exposure time is 700 ms; (b) Laser power is 120 μW~140 μW, exposure time is 1500 ms
    (a)~(h) The bright field images of contact angle and (i) contact angle analysis of microstructures with different sizes
    Fluorescence and SEM images of cells cultured on large-size microstructures after 48 h. (a) Confocal fluorescence images, blue: nuclei stained by DAPI, excitation wavelength is 405 nm, collected wavelength range is 410 nm~460 nm, red: actin stained ActinRed 555, excitation wavelength is 561 nm, collected wavelength range is 570 nm~620 nm; (b) SEM images and (c) magnified SEM images of local details
    Fluorescence and SEM images of cells cultured on small-size microstructures after 48 h. (a)~(d) 3D confocal fluorescence images, blue: nuclei stained by DAPI, excitation wavelength is 405 nm, collected wavelength range is 410 nm~460 nm, red: actin stained ActinRed 555, excitation wavelength is 561 nm, collected wavelength range is 570 nm~620 nm; (e)~(h) SEM images
    Tools

    Get Citation

    Copy Citation Text

    Weicai Zhang, Meiling Zheng, Xianzi Dong, Jie Liu, Feng Jin. Rapid preparation of high-precision hydrogel micropatterns and its induction of cell behavior[J]. Opto-Electronic Engineering, 2022, 49(2): 210336-1

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category:

    Received: Oct. 21, 2021

    Accepted: --

    Published Online: Apr. 6, 2022

    The Author Email: Zheng Meiling (zhengmeiling@mail.ipc.ac.cn)

    DOI:10.12086/oee.2022.210336

    Topics