Spectral imaging is a method that integrates spectral and imaging techniques to extract spatial information and spectral information of an object to form a three-dimensional hypercube. As a fast and nondestructive optical inspection method, spectral imaging can meet the requirements of forensic science for examining physical evidence. Specifically, it plays a crucial role in the appearance, identification, and classification of trace evidence. In this study, the principle and workflow of spectral imaging are introduced, followed by cutting-edge applications in forensic science, such as examinations of document, blood stain, and fingerprint. Furthermore, the dilemma and future trend of spectral imaging are analyzed to promote further technological development that can serve forensic science.