Acta Photonica Sinica, Volume. 51, Issue 6, 0618001(2022)

In Vivo,Dual-color Fluorescent Imaging Miniature Microscope

Kaiqiu LAN1...2,*, Xibin YANG2, Baoteng XU2, Jialin LIU2, Wei ZHOU2 and Daxi XIONG2 |Show fewer author(s)
Author Affiliations
  • 1School of Biomedical Engineering(Suzhou),Division of Life Sciences and Medicine,University of Science and Technology of China,Suzhou,Jiangsu 215163,China
  • 2Center of Light and Health,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou,Jiangsu 215163,China
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    Figures & Tables(13)
    The cross-sectional view of DCFIMM-SBIand DCFIMM-DBI
    Schematic diagram of the lens focusing light of different wavelengths
    Optical path of optical system
    Point diagram and chromatic focal shift of the optical systems
    DCFIMM circuit principle
    Photograph of the DCFIMM imaging experiment
    Results of the USAF resolution target imaging experiment
    Results of the fluorescent microsphere imaging experiment
    Results of the hybrid fluorescent microsphere imaging experiment
    Results of the hybrid fluorescent microsphere focusing and contrast imaging experiment
    • Table 1. The main component of DCFIMM

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      Table 1. The main component of DCFIMM

      ComponentDimension/mmModel
      Outer casing22×17×143D printing(Wenext)
      470 nm-LED3×4.5×2LXML-PB01-0030(Lumileds)
      630 nm-LED3×4.5×2LXM2-PD01-0060(Lumileds)
      Dichroic mirror for combining excitation light5×6×1T495lpxr(Chroma)
      Excitation filter5×5×1ET-Fitc/Cy5x(Chroma)
      Dichroic mirror5×7×1TFITC/Cy5rpc(Chroma)
      Emission filter5×5×1ET-Fitc/Cy5m(Chroma)
      Collimator lens(L1)Diameter 347-269(Edmund)
      Imaging systemSee Table 2 for details
      PCB(including micro SD Card)17×23×4Custom design
      Battery14×10×6601215(Yuhuida)
      Metal sleeve1.2×4(thickness 0.1)Custom design
    • Table 2. The components of DCFIMM imaging optical systems

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      Table 2. The components of DCFIMM imaging optical systems

      Imaging optical systemComponentDiameter /mmFocal length /mmModel
      L2DCFIMM-SBIImaging lens41263-692(Edmund)
      L3Objective lens4663-690(Edmund)
      L43645-089(Edmund)
      L2DCFIMM-DBIImaging lens41263-692(Edmund)
      L5Objective lens1365-566(Edmund)
    • Table 3. The main parameters of DCFIMM imaging optical systems

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      Table 3. The main parameters of DCFIMM imaging optical systems

      Optical systemMagnificationNumerical aperture/(N.A.)Field of view/mm2Work distance/mm
      GRIN lens6.60.400.55×0.550.17
      DCFIMM-SBI3.20.411.10×1.100.60
      DCFIMM-DBI4.20.150.77×0.772.40
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    Kaiqiu LAN, Xibin YANG, Baoteng XU, Jialin LIU, Wei ZHOU, Daxi XIONG. In Vivo,Dual-color Fluorescent Imaging Miniature Microscope[J]. Acta Photonica Sinica, 2022, 51(6): 0618001

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    Paper Information

    Category: Microscopy

    Received: Dec. 16, 2021

    Accepted: Jan. 20, 2022

    Published Online: Sep. 23, 2022

    The Author Email: LAN Kaiqiu (jylankaiqiu@163.com)

    DOI:10.3788/gzxb20225106.0618001

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