Fig. 1. ⁶⁰Co-γ ray and electron beam radiation mutagenesis technology roadmap for Bacillus velezensis

Fig. 2. Effect of absorbed dose on survival of Bacillus velezensis: (a) ⁶⁰Co-γ ray; (b) electron beam

Fig. 3. Correlation curve between viable cell concentration and OD₆₀₀ of Bacillus velezensis

Fig. 4. Growth curve of Bacillus velezensis

Fig. 5. Lethal rate of Bacillus velezensis under different absorbed doses of ⁶⁰Co-γ ray and electron beam irradiation

Fig. 6. Radiation mutagenesis strains antagonistic Colletotrichum gloeosporioides increased amount: (a) ⁶⁰Co-γ ray; (b) electron beam

Fig. 7. Inhibition rate of original strain and radiation mutagenesis strain on Colletotrichum gloeosporioides: (a) ⁶⁰Co-γ ray; (b) electron beam The letters were different from CK, and there was a significant difference compared with CK (p<0.05)

Fig. 8. Bacteriostatic effect of the original strain and the radiation mutagenic strain was determined by the four-point method a: blank control; b: CK; c: B004; d: B112; e: B114; f: B117; g: B118; D1: CK; D2: D115; D3: D243

Fig. 9. Genetic stability of antibacterial effect of ⁶⁰Co-γ ray radiation mutagenesis strains (color online) The letters were different from CK, and there was a significant difference compared with CK (p<0.05)

Fig. 10. Four-point method was used to determine the antibacterial genetic stability of ⁶⁰Co-γ ray irradiated mutant strains a: blank control; b1: CK; c1: B004; d1: B112; e1: B114; f1: B117; g1: B118; b5: CK fifth generation; c5: B004 fifth generation; d5: B112 fifth generation; e5: B114 fifth generation; f5: B117 fifth generation; g5: B118 fifth generation; b6: CK sixth generation; c6: B004 sixth generation; d6: B112 sixth generation; e6: B114 sixth generation; f6: B117 sixth generation; g6: B118 sixth generation