[1] [1] Jonietz E. Mechanics: the forces of cancer. Nature. 2012;491:S56–7.10.1038/491S56a

[2] [2] Panciera T, et al. Reprogramming normal cells into tumour precursors requires ECM stiffness and oncogene-mediated changes of cell mechanical properties. Nat Mater. 2020;19:797–806.10.1038/s41563-020-0615-x

[3] [3] Yuan S, Norgard RJ, Stanger BZ. Cellular plasticity in cancer. Cancer Discov. 2019;9:837–51.10.1158/2159-8290.CD-19-0015

[4] [4] Cross SE, Jin YS, Rao J, Gimzewski JK. Nanomechanical analysis of cells from cancer patients. Nat Nanotech. 2007;2:780–3.10.1038/nnano.2007.388

[5] [5] Han YL, et al. Cell swelling, softening and invasion in a three-dimensional breast cancer model. Nat Phys. 2020;16:101–8.10.1038/s41567-019-0680-8

[6] [6] Lam V, Bigley T, Terhune SS, Wakatsuki T. A method for quantifying mechanical properties of tissue following viral infection. PLoS One. 2012;7:e42197.

[7] [7] Kräter M, et al. Alterations in cell mechanics by actin cytoskeletal changes correlate with strain-specific rubella virus phenotypes for cell migration and induction of apoptosis. Cells. 2018;7:136.

[8] [8] Lampi MC, Reinhart-King CA. Targeting extracellular matrix stiffness to attenuate disease: from molecular mechanisms to clinical trials. Sci Transl Med. 2018;10:eaao0475.10.1126/scitranslmed.aao0475

[9] [9] Phillip JM, Aifuwa I, Walston J, Wirtz D. The mechanobiology of aging. Annu Rev Biomed Eng. 2015;17:113–41.10.1146/annurev-bioeng-071114-040829

[10] [10] Liu B, McNally S, Kilpatrick JI, Jarvis SP, O’Brien CJ. Aging and ocular tissue stiffness in glaucoma. Surv Ophthalmol. 2018;63:56–74.10.1016/j.survophthal.2017.06.007

[11] [11] Overby DR, et al. Altered mechanobiology of Schlemm’s canal endothelial cells in glaucoma. Proc Natl Acad Sci U S A. 2014;111:13876–81.10.1073/pnas.1410602111

[12] [12] Xu W, et al. Cell stiffness is a biomarker of the metastatic potential of ovarian cancer cells. PLoS ONE. 2012;7: e46609.10.1371/journal.pone.0046609

[13] [13] Raudenska M, et al. Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation. Sci Rep. 2019;9:1660.10.1038/s41598-018-38199-7

[14] [14] Huang J, et al. Extracellular matrix and its therapeutic potential for cancer treatment. Sig Transduct Target Ther. 2021;6:1–24.10.1038/s41392-021-00544-0

[15] [15] Cecelja M, Shanahan CM. Targeting cell stiffness. Circul Res. 2021;128:769–71.10.1161/CIRCRESAHA.121.318954

[16] [16] Paluch E, Heisenberg CP. Biology and physics of cell shape changes in development. Curr Biol. 2009;19:R790-799.10.1016/j.cub.2009.07.029

[17] [17] Handorf AM, Zhou Y, Halanski MA, Li W-J. Tissue stiffness dictates development, homeostasis, and disease progression. Organogenesis. 2015;11:1–15.10.1080/15476278.2015.1019687

[18] [18] Nematbakhsh Y, Lim CT. Cell biomechanics and its applications in human disease diagnosis. Acta Mech Sin. 2015;31:268–73.10.1007/s10409-015-0412-y

[19] [19] Quan F-S, Kim KS. Medical applications of the intrinsic mechanical properties of single cells. Acta Biochim Biophys Sin. 2016;48:865–71.10.1093/abbs/gmw081

[20] [20] Guimarães CF, Gasperini L, Marques AP, Reis RL. The stiffness of living tissues and its implications for tissue engineering. Nat Reviews Mater. 2020;5:351–70.10.1038/s41578-019-0169-1

[21] [21] Essmann CL, et al. Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. Elegans. Nat Commun. 2020;11:1043.10.1038/s41467-020-14785-0

[22] [22] Guillou L, Babataheri A, Puech P-H, Barakat AI, Husson J. Dynamic monitoring of cell mechanical properties using profile microindentation. Sci Rep. 2016;6: 21529.

[23] [23] Zhang Y, et al. Interfacing 3D magnetic twisting cytometry with confocal fluorescence microscopy to image force responses in living cells. Nat Protoc. 2017;12:1437–50.10.1038/nprot.2017.042

[24] [24] Herráez-Aguilar D, et al. Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells. Sci Rep. 2020;10:6707.10.1038/s41598-020-63682-5

[25] [25] Guck J, et al. Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. Biophys J. 2005;88:3689–98.10.1529/biophysj.104.045476

[26] [26] Wang S, Larin KV. Optical coherence elastography for tissue characterization: a review. J Biophoton. 2015;8:279–302.10.1002/jbio.201400108

[27] [27] Larin KV, Sampson DD. Optical coherence elastography – OCT at work in tissue biomechanics. Biomed Opt Express. 2017;8:1172–202.10.1364/BOE.8.001172

[28] [28] Meng Z, Traverso AJ, Ballmann CW, Troyanova-Wood MA, Yakovlev V. V. Seeing cells in a new light: a renaissance of Brillouin spectroscopy. Adv Opt Photon. 2016;8:300–27.10.1364/AOP.8.000300

[29] [29] Prevedel R, Diz-Muñoz A, Ruocco G, Antonacci G. Brillouin microscopy: an emerging tool for mechanobiology. Nat Methods. 2019;16:969–77.10.1038/s41592-019-0543-3

[30] [30] Palombo F, Fioretto D. Brillouin light scattering: applications in biomedical sciences. Chem Rev. 2019;119:7833–47.10.1021/acs.chemrev.9b00019

[31] [31] Scarcelli G, et al. Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy. Nat Methods. 2015;12:1132–4.10.1038/nmeth.3616

[32] [32] Pakhomov AG, et al. Disassembly of actin structures by nanosecond pulsed electric field is a downstream effect of cell swelling. Bioelectrochemistry. 2014;100:88–95.10.1016/j.bioelechem.2014.01.004

[33] [33] Carr L, et al. Calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields in U87 human glioblastoma cells. Sci Rep. 2017;7: 41267.10.1038/srep41267

[34] [34] Meng Z, Bustamante Lopez SC, Meissner KE, Yakovlev VV. Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy. J Biophotonics. 2016;9:201–7.10.1002/jbio.201500163

[35] [35] Fiore A, Zhang J, Shao P, Yun SH, Scarcelli G. High-extinction VIPA-based Brillouin spectroscopy of turbid biological media. Appl Phys Lett. 2016;108:1–9.10.1063/1.4948353

[36] [36] Antonacci G, Braakman S. Biomechanics of subcellular structures by non-invasive Brillouin microscopy. Sci Rep. 2016;6:37217.

[37] [37] Mattana S, et al. Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques. Light: Sci Appl. 2018;7:17139–17139.10.1038/lsa.2017.139

[38] [38] Troyanova-Wood M, Meng Z, Yakovlev VV. Differentiating melanoma and healthy tissues based on elasticity-specific Brillouin microspectroscopy. Biomed Opt Express BOE. 2019;10:1774–81.10.1364/BOE.10.001774

[39] [39] Margueritat J, et al. High-frequency mechanical properties of tumors measured by Brillouin light scattering. Phys Rev Lett. 2019;122:018101.

[40] [40] Zhang J, et al. Nuclear mechanics within intact cells is regulated by cytoskeletal network and internal nanostructures. Small. 2020;16:1907688.

[41] [41] Batista Napotnik T, Reberšek M, Vernier PT, Mali B, Miklavčič D. Effects of high voltage nanosecond electric pulses on eukaryotic cells (in vitro): a systematic review. Bioelectrochemistry. 2016;110:1–12.10.1016/j.bioelechem.2016.02.011

[42] [42] Kotnik T, Rems L, Tarek M, Miklavčič D. Membrane electroporation and electropermeabilization: mechanisms and models. Annu Rev Biophys. 2019;48:63–91.10.1146/annurev-biophys-052118-115451

[43] [43] Antonacci G, et al. Recent progress and current opinions in Brillouin microscopy for life science applications. Biophys Rev. 2020;12:615–24.10.1007/s12551-020-00701-9

[44] [44] Thompson GL, Roth C, Tolstykh G, Kuipers M, Ibey BL. Disruption of the actin cortex contributes to susceptibility of mammalian cells to nanosecond pulsed electric fields. Bioelectromagnetics. 2014;35:262–72.10.1002/bem.21845

[45] [45] Tolstykh GP, Thompson GL, Beier HT, Steelman ZA, Ibey BL. nsPEF-induced PIP2 depletion, PLC activity and actin cytoskeletal cortex remodeling are responsible for post-exposure cellular swelling and blebbing. Biochem Biophys Rep. 2017;9:36–41.

[46] [46] Thompson GL, Roth CC, Dalzell DR, Kuipers M, Ibey BL. Calcium influx affects intracellular transport and membrane repair following nanosecond pulsed electric field exposure. J Biomed Opt. 2014;19:055005.10.1117/1.JBO.19.5.055005

[47] [47] Rosenmund C, Westbrook GL. Calcium-induced actin depolymerization reduces NMDA channel activity. Neuron. 1993;10:805–14.10.1016/0896-6273(93)90197-Y

[48] [48] O’Brien ET, Salmon ED, Erickson HP. How calcium causes microtubule depolymerization. Cell Motil. 1997;36:125–35.10.1002/(SICI)1097-0169(1997)36:2<125::AID-CM3>3.0.CO;2-8

[49] [49] Steinkühler J, Sezgin E, Urbančič I, Eggeling C, Dimova R. Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density. Commun Biol. 2019;2:337.10.1038/s42003-019-0583-3

[50] [50] Guck J. Some thoughts on the future of cell mechanics. Biophys Rev. 2019;11:667–70.10.1007/s12551-019-00597-0

[51] [51] Ibey BL, Xiao S, Schoenbach KH, Murphy MR, Pakhomov AG. Plasma membrane permeabilization by 60- and 600-ns electric pulses is determined by the absorbed dose. Bioelectromagnetics. 2009;30:92–9.10.1002/bem.20451

[52] [52] Steelman ZA, Tolstykh GP, Beier HT, Ibey BL. Cellular response to high pulse repetition rate nanosecond pulses varies with fluorescent marker identity. Biochem Biophys Res Commun. 2016;478:1261–7.10.1016/j.bbrc.2016.08.107

[53] [53] Steelman ZA, et al. Quantitative phase microscopy monitors subcellular dynamics in single cells exposed to nanosecond pulsed electric fields. J Biophotonics. 2021;14: e202100125. .10.1002/jbio.202100125

[54] [54] Nesin OM, Pakhomova ON, Xiao S, Pakhomov AG. Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses. Biochim et Biophys Acta (BBA) - Biomembr. 2011;1808:792–801.10.1016/j.bbamem.2010.12.012

[55] [55] Breton M, Mir LM. Microsecond and nanosecond electric pulses in cancer treatments. Bioelectromagnetics. 2012;33:106.10.1002/bem.20692

[56] [56] Xiao D, et al. Irreversible electroporation and apoptosis in human liver cancer cells induced by nanosecond electric pulses. Bioelectromagnetics. 2013;34:512–20.10.1002/bem.21796

[57] [57] Rols M-P, et al. In vivo electrically mediated protein and gene transfer in murine melanoma. Nat Biotechnol. 1998;16:168–71.10.1038/nbt0298-168

[58] [58] Saldaña G, Álvarez I, Condón S, Raso J. Microbiological aspects related to the feasibility of PEF technology for food pasteurization. Crit Rev Food Sci Nutr. 2014;54:1415–26.10.1080/10408398.2011.638995

[59] [59] Caponi S, Fioretto D, Mattarelli M. On the actual spatial resolution of Brillouin imaging. Opt Lett OL. 2020;45:1063–6.10.1364/OL.385072

[60] [60] Antonacci G, Foreman MR, Paterson C, Török P. Spectral broadening in Brillouin imaging. Appl Phys Lett. 2013;103:5–8.10.1063/1.4836477

[61] [61] Zhang J, Scarcelli G. Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes. Nat Protoc. 2021;16:1251–75.10.1038/s41596-020-00457-2

[62] [62] Vincelette RL, et al. Thresholds for phosphatidylserine externalization in Chinese hamster ovarian cells following exposure to nanosecond pulsed electrical fields (nsPEF). PLoS ONE. 2013;8:e63122.

[63] [63] Nikolić M, Scarcelli G. Long-term Brillouin imaging of live cells with reduced absorption-mediated damage at 660nm wavelength. Biomed Opt Express BOE. 2019;10:1567–80.10.1364/BOE.10.001567

[64] [64] Alisafaei F, Jokhun DS, Shivashankar GV, Shenoy VB. Regulation of nuclear architecture, mechanics, and nucleocytoplasmic shuttling of epigenetic factors by cell geometric constraints. Proc Natl Acad Sci USA. 2019;116:13200–9.10.1073/pnas.1902035116

[65] [65] Steelman ZA, et al. Comprehensive single-shot biophysical cytometry using simultaneous quantitative phase imaging and Brillouin spectroscopy. Sci Rep. 2022;12:18285.10.1038/s41598-022-23049-4

[66] [66] Troyanova-Wood M, Gobbell C, Meng Z, Gashev AA, Yakovlev VV. Optical assessment of changes in mechanical and chemical properties of adipose tissue in diet-induced obese rats. J Biophotonics. 2017;9:1–10.

[67] [67] Traverso AJ, et al. Dual Raman-Brillouin microscope for chemical and mechanical characterization and imaging. Anal Chem. 2015;87:7519–23.10.1021/acs.analchem.5b02104

[68] [68] Steelman Z, Meng Z, Traverso AJ, Yakovlev VV. Brillouin spectroscopy as a new method of screening for increased CSF total protein during bacterial meningitis. J Biophotonics. 2015;8:408–14.10.1002/jbio.201400047

[69] [69] Coker Z, et al. Assessing performance of modern Brillouin spectrometers. Opt Express. 2018;26:2400.

[70] [70] Meng Z, Yakovlev VV. Optimizing signal collection efficiency of the VIPA-based Brillouin spectrometer. J Innov Opt Health Sci. 2014;08:1550021.10.1142/S1793545815500212

[71] [71] Roth CC, et al. Characterization of pressure transients generated by nanosecond electrical pulse (nsEP) exposure. Sci Rep. 2015;5: 15063.10.1038/srep15063

[72] [72] Lieber CA, Mahadevan-Jansen A. Automated method for subtraction of fluorescence from biological Raman spectra. Appl Spectrosc. 2003;57:1363–7.10.1366/000370203322554518