Journal of Radiation Research and Radiation Processing, Volume. 41, Issue 5, 050303(2023)
Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components
Figures & Tables(12)
Fig. 3. Comparison fingerprint of various components of radiation samples
Table 1. Gradient elution procedure
View tableView in ArticleTable 1. Gradient elution procedure
时间 / min Time 流速 /（mL∙min-1）Velocity 乙腈 / % Acetonitrile 0.5%冰醋酸 / % Glacial acetic acid 0.00 1.00 10.0 90.0 15.00 1.00 10.0 90.0 30.00 1.00 20.0 80.0 35.00 1.00 30.0 70.0 40.00 1.00 55.0 45.0 45.00 1.00 55.0 45.0 55.00 1.00 95.0 5.0 60.00 1.00 95.0 5.0 65.00 1.00 10.0 90.0 70.00 1.00 10.0 90.0
Table 2. Linearity range of each group
View tableView in ArticleTable 2. Linearity range of each group
序号Serial
Number
组分
Components
出峰时间 / min
Peak time
判定系数
Judgment coefficient
线性范围 /（μg∙mL-1）
Linear range
1 绿原酸Chlorogenic acid 11.807~11.920 0.997 83 12.26~61.30 2 川芎嗪Ligustrazine 14.780~15.093 0.999 70 19.60~98.00 3 阿魏酸Ferulic acid 31.520~31.597 0.999 85 9.86~49.28 4 阿魏酸松柏酯Coniferly ferulate 45.520~45.647 0.999 75 10.00~50.00 5 洋川芎内酯Senkyunolide 48.820~48.857 0.999 60 100.00~500.00 6 正丁基苯酚N-butyl phenol 49.470~49.507 0.999 97 10.00~50.00 7 藁本内酯Ligustilide 51.950~51.967 0.999 99 100.00~500.00 8 欧当归内酯Levistilide 56.410~56.413 0.999 95 16.50~82.50
Table 3. Experimental results of spiked recovery rate
View tableView in ArticleTable 3. Experimental results of spiked recovery rate
组分
Components
低浓度
/ (μg∙mL-1)
Low concentration
实测浓度
/ (μg∙mL-1)Measured concentration
回收率 / %
Rate of recovery
中浓度
/ (μg∙mL-1)
Medium concentration
实测浓度
/ (μg∙mL-1)Measured concentration
回收率 / %
Rate of recovery
高浓度
/ (μg∙mL-1)
High concentration
实测浓度
/ (μg∙mL-1)Measured concentration
回收率 / %
Rate of recovery
绿原酸Chlorogenic acid
18.36 17.12 93.25 147.12 134.48 91.41 588.48 539.63 91.70 17.56 95.64 140.70 95.64 564.45 95.92 18.41 100.32 144.13 97.97 547.32 93.01 川芎嗪Ligustrazine
29.40 27.34 92.99 235.20 217.68 92.55 940.80 854.88 90.87 28.70 97.62 225.91 96.05 910.12 96.74 26.65 90.65 227.13 96.57 890.45 94.65 阿魏酸Ferulic acid
14.76 14.30 96.72 118.26 108.43 91.69 473.04 452.76 95.71 13.63 92.19 110.86 93.74 430.33 90.97 14.31 96.79 114.08 96.47 447.34 94.57 阿魏酸松柏Coniferly ferulate
15.00 13.76 91.73 120.00 123.11 102.59 480.00 452.35 94.24 15.35 102.33 108.97 90.81 471.33 98.19 14.70 98.00 117.38 97.82 467.42 97.38 洋川芎内酯Senkyunolide
15.00 15.64 104.27 120.00 120.61 100.51 480.00 465.12 96.90 14.43 96.23 124.10 103.42 475.87 99.14 14.73 98.17 123.36 102.80 470.40 98.00 正丁基苯酚N-butyl phenol
15.00 14.95 99.67 120.00 119.37 99.48 480.00 498.81 103.92 15.11 100.73 118.80 99.00 481.50 100.31 14.64 97.60 117.52 97.93 486.17 101.29 藁本内酯Ligustilide
15.00 14.25 95.00 120.00 117.27 97.73 480.00 460.92 96.03 14.74 98.27 115.38 96.15 456.12 95.03 14.47 96.47 115.50 96.25 451.39 94.04 欧当归内酯Levistilide
24.75 22.75 91.92 198.00 183.39 92.62 792.00 769.23 97.13 23.22 93.82 181.40 91.62 780.69 98.57 23.51 94.99 179.18 90.49 754.86 95.31
Table 4.
Precision measurement results of 8 compounds View tableView in ArticleTable 4.
Precision measurement results of 8 compounds 组分
Components
第1次
First time
第2次
Second time
第3次
Third time
第4次Fourth time 第5次
Fifth time
第6次
Sixth time
平均值
Average value
RSD / % 绿原酸
Chlorogenic acid
0.544 3 0.530 2 0.512 1 0.513 4 0.512 5 0.514 2 0.521 1 2.55 川芎嗪
Ligustrazine
0.041 0 0.041 3 0.041 3 0.041 1 0.041 2 0.041 3 0.041 2 0.27 阿魏酸
Ferulic acid
1.015 3 1.095 2 1.078 5 1.016 1 1.173 3 1.167 6 1.091 0 0.64 阿魏酸松柏酯
Coniferly ferulate
0.031 2 0.031 2 0.031 2 0.031 3 0.031 2 0.031 3 0.031 2 0.07 洋川芎内酯
Senkyunolide
1.323 4 1.322 5 1.321 1 1.321 0 1.321 4 1.321 0 1.321 7 0.08 正丁基苯酚
N-butyl phenol
0.023 1 0.023 2 0.023 1 0.023 2 0.023 1 0.023 2 0.023 2 0.27 藁本内酯
Ligustilide
4.432 0 4.428 0 4.436 0 4.426 0 4.422 0 4.424 0 4.428 0 0.12 欧当归内酯
Levistilide
0.122 0 0.121 8 0.122 6 0.122 9 0.121 8 0.122 1 0.122 2 0.37
Table 5. Stability results of 8 compounds
View tableView in ArticleTable 5. Stability results of 8 compounds
组分
Components
0 h 2 h 4 h 8 h 12 h 平均值
Average value
RSD / % 绿原酸
Chlorogenic acid
0.561 2 0.548 7 0.546 6 0.559 6 0.529 7 0.549 2 2.30 川芎嗪
Ligustrazine
0.043 3 0.046 4 0.045 3 0.043 8 0.044 6 0.044 7 2.75 阿魏酸
Ferulic acid
1.024 5 1.033 6 1.024 8 1.025 2 1.033 0 1.028 2 0.45 阿魏酸松柏酯
Coniferly ferulate
0.046 5 0.047 8 0.048 1 0.046 5 0.048 7 0.047 5 2.07 洋川芎内酯
Senkyunolide
1.350 2 1.329 7 1.353 6 1.348 6 1.332 7 1.343 0 0.81 正丁基苯酚
N-butyl phenol
0.037 4 0.035 3 0.038 2 0.036 9 0.037 0 0.037 0 2.87 藁本内酯
Ligustilide
4.431 0 4.428 0 4.431 0 4.410 7 4.428 2 4.425 8 0.19 欧当归内酯
Levistilide
0.143 2 0.145 8 0.138 0 0.140 1 0.140 8 0.141 6 2.12
Table 6. Repetitive results of 8 compounds
View tableView in ArticleTable 6. Repetitive results of 8 compounds
组分
Components
1 2 3 4 5 6 平均值
Average value
RSD / % 绿原酸
Chlorogenic acid
0.521 5 0.511 8 0.546 8 0.529 1 0.523 8 0.530 2 0.527 2 2.21 川芎嗪
Ligustrazine
0.044 6 0.046 0 0.046 5 0.044 3 0.045 5 0.045 8 0.045 5 1.86 阿魏酸
Ferulic acid
0.993 2 1.028 4 1.036 5 0.995 3 1.008 3 1.011 4 1.012 2 1.72 阿魏酸松柏酯
Coniferly ferulate
0.034 6 0.034 7 0.034 3 0.034 8 0.035 2 0.035 1 0.034 8 0.92 洋川芎内酯
Senkyunolide
1.235 8 1.269 1 1.312 0 1.322 5 1.288 9 1.336 8 1.294 2 2.89 正丁基苯酚
N-butyl phenol
0.028 9 0.028 5 0.029 9 0.030 3 0.029 8 0.029 4 0.029 5 2.29 藁本内酯
Ligustilide
4.468 9 4.418 1 4.434 7 4.429 6 4.423 8 4.435 4 4.435 1 0.40 欧当归内酯
Levistilide
0.133 3 0.131 7 0.132 4 0.129 7 0.132 8 0.133 2 0.132 2 1.02
Table 7. Radiation sample similarity
View tableView in ArticleTable 7. Radiation sample similarity
S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 S12 S13 S14 S15 R S1 1.000 0.995 0.996 0.994 0.996 0.997 0.990 0.993 0.993 0.994 0.994 0.995 0.988 0.990 0.993 0.996 S2 0.995 1.000 0.992 0.996 0.997 0.996 0.992 0.995 0.994 0.994 0.994 0.995 0.992 0.992 0.994 0.997 S3 0.996 0.992 1.000 0.995 0.997 0.998 0.995 0.994 0.996 0.996 0.996 0.996 0.991 0.993 0.995 0.997 S4 0.994 0.996 0.995 1.000 0.999 0.999 0.998 0.999 0.999 0.998 0.999 0.998 0.998 0.998 0.999 0.999 S5 0.996 0.997 0.997 0.998 1.000 1.000 0.997 0.998 0.998 0.998 0.998 0.999 0.995 0.996 0.998 0.999 S6 0.997 0.996 0.998 0.999 1.000 1.000 0.996 0.997 0.998 0.998 0.998 0.999 0.994 0.996 0.997 0.999 S7 0.990 0.992 0.995 0.999 0.997 0.996 1.000 0.999 1.000 0.999 0.999 0.998 0.999 1.000 0.999 0.998 S8 0.993 0.995 0.994 0.998 0.998 0.997 0.999 1.000 1.000 1.000 1.000 0.999 0.999 0.999 1.000 0.999 S9 0.993 0.994 0.996 0.999 0.998 0.998 1.000 1.000 1.000 1.000 1.000 0.999 0.998 0.999 1.000 0.999 S10 0.994 0.994 0.996 0.998 0.998 0.998 0.999 1.000 1.000 1.000 1.000 1.000 0.998 0.999 1.000 0.999 S11 0.994 0.994 0.996 0.999 0.998 0.998 0.999 1.000 1.000 1.000 1.000 1.000 0.998 0.999 1.000 0.999 S12 0.995 0.995 0.996 0.998 0.999 0.999 0.998 0.999 0.999 1.000 1.000 1.000 0.996 0.998 0.999 0.999 S13 0.988 0.992 0.991 0.998 0.995 0.994 0.999 0.999 0.998 0.998 0.998 0.996 1.000 0.999 0.999 0.997 S14 0.990 0.992 0.993 0.998 0.996 0.996 1.000 0.999 0.999 0.999 0.999 0.998 0.999 1.000 1.000 0.998 S15 0.993 0.994 0.995 0.999 0.998 0.997 0.999 1.000 1.000 1.000 1.000 0.999 0.999 1.000 1.000 0.999 R 0.996 0.997 0.997 0.999 0.999 0.999 0.998 0.999 0.999 0.999 0.999 0.999 0.997 0.998 0.999 1.000
Table 8.
Results of content determination of various constituents under different 60Co-γ radiation dose View tableView in ArticleTable 8.
Results of content determination of various constituents under different 60Co-γ radiation dose 吸收剂量 / kGyAbsorbed dose
绿原酸Chlorogenic acid
川芎嗪Ligustrazine
阿魏酸Ferulic acid
阿魏酸松柏酯 Coniferly ferulate
洋川芎内酯Senkyunolide
正丁基苯酚 N-butyl phenol
藁本内酯Ligustilide
欧当归内酯Levistilide
0 4.21 0.10 1.82 2.21 14.47 0.24 27.86 0.23 0 4.07 0.09 1.67 2.02 13.30 0.22 25.65 0.21 0 3.96 0.09 1.62 2.06 13.08 0.21 25.17 0.21 平均含量±SD
Average content±SD
4.08±0.12 0.09±0.01 1.70±0.10 2.10±0.10 13.62±0.75 0.22±0.01 26.23±1.43 0.22±0.01 3 3.55 0.09 1.90 1.88 14.20 0.25 26.18 0.21 3 3.64 0.09 2.14 1.96 14.04 0.25 25.81 0.21 3 3.89 0.09 2.28 1.72 14.28 0.25 27.87 0.23 平均含量±SD
Average content±SD
3.69±0.18 0.09±0.00 2.10±0.19 1.85±0.12 14.17±012 0.25±0.00 26.62±1.10 0.22±0.01 6 4.86 0.14 1.82 2.09 14.11 0.26 27.52 0.21 6 3.69 0.09 1.91 1.90 14.22 0.26 25.92 0.26 6 4.13 0.13 1.97 1.85 14.06 0.29 27.34 0.28 平均含量±SD
Average content±SD
4.23±0.59 0.12±0.03 1.90±0.08 1.95±0.13 14.13±0.08 0.27±0.02 26.93±0.88 0.25±0.04 10 2.17 0.05 1.11 1.93 7.84 0.15 15.37 0.16 10 3.53 0.08 1.73 1.60 12.89 0.26 24.62 0.25 10 3.37 0.09 1.90 1.23 12.54 0.25 23.59 0.24 平均含量±SD
Average content±SD
3.03±0.74 0.07±0.02 1.58±0.41 1.59±0.35 11.09±2.82 0.22±0.06 21.19±5.07 0.22±0.05 15 3.74 0.09 1.63 2.60 14.37 0.29 26.44 0.27 15 3.87 0.12 1.56 2.06 12.66 0.29 24.00 0.25 15 3.93 0.10 1.87 1.86 13.97 0.28 26.78 0.28 平均含量±SD
Average content±SD
3.85±0.10 0.11±0.02 1.68±0.16 2.17±0.38 13.67±0.90 0.29±0.002 25.74±1.52 0.27±0.01
Table 9.
Significance probability value of t test View tableView in ArticleTable 9.
Significance probability value of t test 成分Component 不同吸收剂量下的显著性概率值（n=3）
Probability value of significance under difference absorbed dose
3 kGy 6 kGy 10 kGy 15 kGy 绿原酸Chlorogenic acid 0.305 0.690 0.014（＜0.05） 0.529 川芎嗪Ligustrazine 0.812 0.080 0.172 0.481 阿魏酸Ferulic acid 0.053 0.309 0.517 0.929 阿魏酸松柏酯Coniferly ferulate 0.259 0.478 0.031（＜0.05） 0.714 洋川芎内酯Senkyunolide 0.629 0.655 0.047（＜0.05） 0.965 正丁基苯酚N-butyl phenol 0.290 0.079 0.892 0.024（＜0.05） 藁本内酯Ligustilide 0.853 0.742 0.035（＜0.05） 0.819 欧 当归内酯Levistilide1.000 0.191 1.000 0.062
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Yalu QIU, Peng GAO, Jiang HE, Qian CHEN, Yan WANG. Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components[J]. Journal of Radiation Research and Radiation Processing, 2023, 41(5): 050303
Paper Information
Category: Research Articles
Received: Feb. 20, 2023
Accepted: Apr. 20, 2023
Published Online: Dec. 27, 2023
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