Fig. 1. Classical LSFM path. (a) SPIM implementation^[5]; (b) OCPI microscope schematic^[11]; (c) DSLM implementation^[5]

Fig. 2. Simplified schematic of the Bessel beam plane illumination microscope^[9]

Fig. 3. Schematic of the lattice light sheet microscopy optical path^[10]

Fig. 4. Schematic drawing of light-sheet generation optical path with field synthesis^[13]

Fig. 5. Working principle of the TLS-SPIM^[20]

Fig. 6. Schematic of the swept confocally-aligned planar excitation microscopy^[24]

Fig. 7. Protein model^[30]. (a) The HaloTag protein; (b) The ligand tunnel

Fig. 8. Tissue transparency. (a) Cleared mouse brain^[43]; Human brain coronal hemisphere before (b) and after (c) SHIELD processing^[44]

Fig. 9. Observation on the development of zebrafish^[49]. (a) Schematic of the mounting in 1.5% agarose; (b) Schematic of the multilayer mounting in 0.1% agarose in coated FEP tubes; (c) Developing zebrafish embryo